Novel, sustainable approaches for controlling fungal pathogens

控制真菌病原体的新颖、可持续的方法

• 批准号: 2116124
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2116124
• 关键词: Novel; sustainable; approaches; controlling; fungal

This project will investigate natural anti-fungal inhibitors and assess synergistic/antagonistic interactions with other agents, extending to mode-of-action characterization and the impact of environment or media-type on interactions. This will include tests to find agents to which drug resistant fungal isolates may be hypersensitive, e.g., 'collateral sensitivity' in azole-resistant strains of C. albicans. In addition, the project will investigate fungal anti-attachment polymers as a novel, passive means for controlling problematic fungi, potentially encompassing both human- and crop-pathogens. Experiments will use experimental evolution experiments and/or selection after UV mutagenesis in S. cerevisiae, followed by genetic analysis (bulk segregant analysis) or NGS, to reveal mechanisms of anti-attachment and resistance.

该项目将研究天然抗真菌抑制剂，并评估与其他药物的协同/拮抗相互作用，扩展到作用模式表征以及环境或介质类型对相互作用的影响。这将包括测试，以发现药物耐药真菌分离株可能是过敏，例如，“间接敏感性”在唑类耐药菌株的C。白色念珠菌。此外，该项目还将研究真菌抗附着聚合物作为一种新型的被动手段来控制有问题的真菌，可能包括人类和作物病原体。实验将使用实验进化实验和/或在S.酿酒酵母，然后进行遗传分析（批量分离子分析）或NGS，以揭示抗附着和抗性的机制。