Mechanisms of Salivary and Lacrimal Inflammation

唾液和泪道炎症的机制

• 批准号: 6620720
• 负责人: SARA A MICHIE
• 金额: $ 24.05万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2005-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6620720
• 关键词: B lymphocyte; NOD mouse; Sjogren's syndrome; T lymphocyte; cell migration; chemokine; cytokine receptors; disease /disorder model; flow cytometry; fluorescence microscopy; gene expression; gene targeting; genetically modified animals; immunocytochemistry; immunofluorescence technique; immunotherapy; inflammation; lacrimal apparatus; laser capture microdissection; leukocyte activation /transformation; monoclonal antibody; polymerase chain reaction; protein structure function; receptor expression; salivary glands; vascular cell adhesion molecule; vascular endothelium

DESCRIPTION: The migration of autoreactive and effector lymphocytes from blood into salivary and lacrimal glands is a key event in the initiation and maintenance of glandular inflammation and damage in Sjogren?s syndrome. This migration involves a multi-step cascade with sequential lymphocyte/endothelial adhesion and activation events. The goals are to identify lymphocyte and endothelial adhesion molecules, chemokines, and chemokine receptors that are expressed in inflamed salivary and lacrimal glands, and to define the roles of these molecules in lymphocyte migration to these tissues. Non-obese diabetic (NOD) mice, a well-characterized model for human Sjogren?s syndrome, will be used for these studies. Specific Aim 1 will define the expression of adhesion molecules, chemokines, and chemokine receptors in inflamed salivary and lacrimal glands from NOD mice. The roles of these adhesion molecules and chemokines (and their receptors) in lymphocyte migration to inflamed glands will be assessed in functional studies in Specific Aim 2. Specific Aim 3 will determine if treatment of NOD mice with antibodies directed against adhesion molecules or chemokines defined in Aims 1 and 2 will prevent the development of salivary and lacrimal gland inflammation.

描述：血液中自身反应性和效应性淋巴细胞的迁移