Shared Microarray Facility

共享微阵列设施

• 批准号: 6667245
• 负责人: MASON W FREEMAN
• 金额: $ 78.2万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6667245
• 关键词: biomedical facility; blood; complementary DNA; disease /disorder; experimental designs; genes; health science research; heart; immunocytochemistry; lung; microarray technology; molecular cloning; oligonucleotides; polymerase chain reaction; statistics /biometry; university

DESCRIPTION (provided by applicant): This grant application proposes to expand and extend the services of the Massachusetts General Hospital Microarray Facility to investigators within the greater Boston community who are supported by grants awarded by the National Heart Lung Blood Institute. Although microarray analysis has opened up the potential to explore the interactions of large numbers of genes simultaneously, the obstacles to successful execution of this work are substantial. One of the chief hurdles to microarray analysis has been cost. Given the imperative to repeat assays in order to ensure reproducibility, this cost has made it difficult, if not impossible, for the typical academic lab to afford to conduct complex microarray studies. By using oligonucleotides synthesized by the MGH Oligonucleotide Facility and arraying our own oligos, we expect to lower substantially this cost barrier. Other factors that have impeded progress in microarray work have included problems with clone/gene availability and/or contamination when using spotted cDNAs, lack of probe or array reproducibility, and insufficient access to bioinformatics expertise in the analysis of very large data sets. Through our commitment to the use of spotted oligonucleotides as the probe sequences on microarrays, the issue of clone availability and contamination should be resolved. Bioinformatics as well as biological solutions to the problem of array reproducibility have been addressed in this grant application. Finally, with the recruitment of several leading local biostatisticians and bioinformaticians as co-investigators or consultants, the MGH Microarray Facility has assembled the requisite expertise and effort to facilitate a collaborating investigator's ability to analyze the large data sets generated by microarray techniques. The resources of this grant will be devoted to supporting the extensive community of NHLBI funded investigators at the major teaching hospitals and universities in the Boston area, including many younger investigators who have not previously had access to this important but costly research tool.

描述（由申请人提供）： 该赠款申请建议将马萨诸塞州综合医院微阵列设施的服务扩展和扩展到大波士顿社区中的调查人员，这些机构得到了国家心脏肺血液研究所授予的赠款的支持。尽管微阵列分析已经开辟了同时探索大量基因相互作用的潜力，但成功执行这项工作的障碍是巨大的。微阵列分析的主要障碍之一是成本。考虑到必须重复测定以确保可重复性的必要性，因此，对于典型的学术实验室而言，这一成本使得很难（即使不是不可能）进行复杂的微阵列研究。通过使用由MGH寡核苷酸设施合成的寡核苷酸并使用我们自己的寡核苷酸合成，我们希望将这种成本障碍大大降低。在使用斑点CDNA，缺乏探针或阵列可重复性的情况下，在微阵列工作中阻碍了微阵列工作的其他因素还包括克隆/基因可用性和/或污染的问题，以及在分析非常大的数据集时无法获得生物信息学专业知识。通过我们对使用斑点寡核苷酸作为微阵列上的探针序列的承诺，应解决克隆可用性和污染的问题。本赠款应用程序已经解决了生物信息学以及针对阵列可重复性问题的生物解决方案。最后，随着几位当地领先的生物统计学家和生物信息学家担任共同评估者或顾问，MGH微阵列设施汇集了必要的专业知识和努力，以促进合作研究者分析由微阵列技术生成的大型数据集的能力。这笔赠款的资源将致力于支持波士顿地区主要教学医院和大学的NHLBI资助的广泛的研究人员，其中包括许多以前没有使用该重要但昂贵的研究工具的年轻调查员。