Endothelial Specific Caveolin-1 Overexpression

描述(申请人提供)：本申请中提出的方案建议使用内皮特异性小窝蛋白-1转基因小鼠来研究小窝蛋白-1在血管功能中的作用。目的1研究转基因小鼠的生物学特性，包括转基因小鼠的组织分布、小窝蛋白-1过表达对组织形态、血压、血管收缩药和血管扩张剂反应的影响。在目标2中，我建议研究从转基因小鼠中分离的内皮细胞。将进行各种血管内皮细胞功能的体外检测，包括细胞增殖、迁移和管样结构形成的检测。目的研究血管内皮细胞特异性小窝蛋白-1过表达对血管通透性和血管生成的影响。总之，本文提出的实验将开发一种新的模型(内皮特异性小窝蛋白-1转基因小鼠)来研究内皮小窝蛋白-1在血流动力学、血管控制、血管通透性和血管生成中的作用。

DESCRIPTION (provided by applicant):The proposal put forth in this application proposes the use of endothelial specific caveolin- 1 transgenic mice to study the role of caveolin-1 in vascular function. Aim 1 centers around the characterization of the transgenic mice, including determination of the tissue distribution of the transgene, and the effect of caveolin-1 overexpression on tissue morphology, blood pressure, the response of isolated blood vessels to vasoconstrictors and vasodilators. In aim 2, I propose to study endothelial cells isolated from the transgenic mice. Various in vitro assays of endothelial cell function will be performed including assays of cell proliferation, migration, and formation of tubule like structures. Aim 3 proposes to study the effect of endothelial specific caveolin-1 overexpression on vascular permeability and angiogenesis in vivo. In summary, the experiments proposed herein will develop a novel model (endothelial specific caveolin-1 transgenic mice) to study the role of endothelial caveolin-1 in hemodynamics, vascular control, vascular permeability, and angiogenesis.