Endothelial Specific Caveolin-1 Overexpression

内皮特异性 Caveolin-1 过表达

• 批准号: 6585367
• 负责人: PHILIP M BAUER
• 金额: $ 4.64万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-30 至 2004-07-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6585367
• 关键词: angiogenesis; biosynthesis; blood pressure; caveolins; cell migration; cell proliferation; gene expression; genetically modified animals; hemodynamics; laboratory mouse; morphology; nitric oxide; protein localization; tissue /cell culture; vascular endothelium permeability

DESCRIPTION (provided by applicant):The proposal put forth in this application proposes the use of endothelial specific caveolin- 1 transgenic mice to study the role of caveolin-1 in vascular function. Aim 1 centers around the characterization of the transgenic mice, including determination of the tissue distribution of the transgene, and the effect of caveolin-1 overexpression on tissue morphology, blood pressure, the response of isolated blood vessels to vasoconstrictors and vasodilators. In aim 2, I propose to study endothelial cells isolated from the transgenic mice. Various in vitro assays of endothelial cell function will be performed including assays of cell proliferation, migration, and formation of tubule like structures. Aim 3 proposes to study the effect of endothelial specific caveolin-1 overexpression on vascular permeability and angiogenesis in vivo. In summary, the experiments proposed herein will develop a novel model (endothelial specific caveolin-1 transgenic mice) to study the role of endothelial caveolin-1 in hemodynamics, vascular control, vascular permeability, and angiogenesis.

描述（由申请人提供）：本申请中提出的建议提出使用内皮特异性小窝蛋白-1转基因小鼠来研究小窝蛋白-1在血管功能中的作用。目的1：研究转基因小鼠的组织分布，caveolin-1过表达对组织形态学、血压、离体血管对血管收缩剂和血管舒张剂的反应的影响。目的2：研究从转基因小鼠中分离的内皮细胞。将进行内皮细胞功能的各种体外测定，包括细胞增殖、迁移和小管样结构形成的测定。目的3研究内皮特异性小窝蛋白-1过表达对血管通透性和血管生成的影响。总之，本文提出的实验将开发一种新的模型（内皮特异性小窝蛋白-1转基因小鼠）来研究内皮小窝蛋白-1在血液动力学、血管控制、血管通透性和血管生成中的作用。