Amygdala Modulation of Hippocampal Arc Expression

描述（由申请人提供）：基底外侧受累 杏仁核（BLA）在情绪影响记忆中的作用已经被人们所知近40年了。 年药理学神经生理学和解剖学证据表明 BLA调节海马系统的信息处理 （包括海马体、齿状回、下托和内嗅 在巩固受情绪影响的记忆过程中。的特定 然而，BLA调节的机制仍不清楚。因为表达 即时早期基因，如Arc和zif 268，在记忆获得过程中， 正常的记忆巩固所必需的，可能是BLA活动， 在记忆获得过程中影响训练诱导的Arc表达， 海马系统中Arc蛋白的水平。这个实验 一项提案将利用荧光技术的最新进展来检验这一假设。 原位杂交方法，以及传统的免疫组织化学和 免疫印迹分析。训练诱导海马Arc表达的变化 在BLA活性被蝇蕈醇或 氧化震颤素，分别降低或增加BLA活性的处理， 并且已经显示出调节受情绪影响的记忆的巩固。 此外，我将研究在记忆过程中改变BLA活动的影响， 记忆过程中海马Arc表达的获得/巩固 检索

DESCRIPTION (provided by applicant): The involvement of the basolateral amygdala (BLA) in emotionally-influenced memory has been known for nearly 40 years. Pharmacological, neurophysiological and anatomical evidence suggests that the BLA modulates information processing in the hippocampal system (comprised of the hippocampus proper, dentate gyrus, subiculum and entorhinal cortex) during consolidation of emotionally-influenced memory. The particular mechanisms of BLA modulation, however, remain unclear. Because expression of immediate early genes, such as Arc and zif268, during memory acquisition is necessary for normal memory consolidation, it is possible that BLA activity during memory acquisition influences training-induced Arc expression and the levels of Arc protein in the hippocampal system. The experiments in this proposal will test this hypothesis utilizing recent advances in fluorescent in situ hybridization methods, as well as traditional immunohistochemistry and immunoblot analyses. Changes in training-induced Arc expression in hippocampal neurons will be examined after BLA activity is altered by either muscimol or oxotremorine, treatments that decrease or increase BLA activity, respectively, and have been shown to modulate consolidation of emotionally-influenced memory. Additionally, I will examine the effect of altered BLA activity during memory acquisition/consolidation on hippocampal Arc expression during memory retrieval.