Amygdala Modulation of Hippocampal Arc Expression

描述(申请人提供)：基侧的参与 杏仁核(BLA)在情绪影响的记忆中已经被知道了近40年 好几年了。药理学、神经生理学和解剖学证据表明 血乳酸对海马区信息处理的调节作用 (由海马体、齿状回、下丘脑和内嗅器组成 在巩固受情绪影响的记忆的过程中。特别之处 然而，BLA的调节机制仍不清楚。因为表达了 记忆获得过程中的即刻早期基因，如Arc和Zif268 对于正常的记忆巩固是必要的，有可能BLA活动 记忆习得对训练诱导的Arc表达的影响 海马系统中Arc蛋白的水平。这里面的实验 一项提案将利用荧光技术的最新进展来检验这一假说 原位杂交方法，以及传统的免疫组织化学和 免疫印迹分析。运动诱导大鼠海马区Arc表达的变化 在血乳酸活性被蝇毒酚或其他药物改变后，对神经元进行检查 分别降低或增加血乳酸活性的治疗， 并已被证明可以调节受情绪影响的记忆的巩固。 此外，我将研究记忆过程中血乳酸活动变化的影响 记忆过程中海马区Arc表达的习得/巩固 取回。

DESCRIPTION (provided by applicant): The involvement of the basolateral amygdala (BLA) in emotionally-influenced memory has been known for nearly 40 years. Pharmacological, neurophysiological and anatomical evidence suggests that the BLA modulates information processing in the hippocampal system (comprised of the hippocampus proper, dentate gyrus, subiculum and entorhinal cortex) during consolidation of emotionally-influenced memory. The particular mechanisms of BLA modulation, however, remain unclear. Because expression of immediate early genes, such as Arc and zif268, during memory acquisition is necessary for normal memory consolidation, it is possible that BLA activity during memory acquisition influences training-induced Arc expression and the levels of Arc protein in the hippocampal system. The experiments in this proposal will test this hypothesis utilizing recent advances in fluorescent in situ hybridization methods, as well as traditional immunohistochemistry and immunoblot analyses. Changes in training-induced Arc expression in hippocampal neurons will be examined after BLA activity is altered by either muscimol or oxotremorine, treatments that decrease or increase BLA activity, respectively, and have been shown to modulate consolidation of emotionally-influenced memory. Additionally, I will examine the effect of altered BLA activity during memory acquisition/consolidation on hippocampal Arc expression during memory retrieval.