Brain Injury and Intercelluar Calcium Waves
脑损伤和细胞间钙波
基本信息
- 批准号:6622425
- 负责人:
- 金额:$ 4.64万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-06-01 至
- 项目状态:未结题
- 来源:
- 关键词:Animalia astrocytes brain injury calcium flux calcium indicator cell cell interaction fluorescence microscopy gap junctions gene expression membrane channels neural transmission neurogenetics neuropharmacologic agent neuropharmacology neuroregulation phosphorylation postdoctoral investigator protein structure function time resolved data western blottings
项目摘要
DESCRIPTION (provided by applicant): The goal of the current proposal is to
evaluate the hypothesis that traumatic brain injury alters intercellular
communication between astrocytes, a potentially crucial and often overlooked
aspect of brain processing. Astrocytes were once viewed as passive cells, but
are now characterized as active contributors to signal processing in the brain.
Yet, the effect of traumatic injury on calcium-mediated astrocyte intercellular
signaling has not been evaluated. Therefore, the proposal evaluates the
hypothesis that traumatic injury alters intercellular calcium signaling between
astrocytes in the glial syncytium using the following specific aims:
Specific Aim 1: Injury of cortical astrocytes alters origination and
propagation of intercellular calcium waves. We will compare the velocity and
distance of intercellular calcium waves in injured astrocytes to that of
uninjured astrocytes. (Method: Use quantitative fluorescent microscopy to
evaluate intercellular calcium waves).
Specific Aim 2: Injury alters gap junction coupling. We will compare
fluorescent dye transfer through gap junctions, expression of Connexin 43
protein, and phosphorylation of Connexin 43 protein between uninjured and
injured astrocytes. (Method: Use lucifer yellow transfer to measure coupling of
gap junctions; use Western Blots to measure expression and phosphorylation of
Connexin 43 protein).
Specific Aim 3: Intercellular calcium signaling will be maintained in
astrocytes by pharmacological manipulation of IP3-mediated intracellular
calcium signaling. (Method: Use agonists and antagonists of elements in the
signaling pathway to determine if alterations in intercellular calcium waves
can be attenuated).
These proposed experiments evaluate, for the first time, the effect of
traumatic brain injury on the intricate and extensive calcium signaling network
among astrocytes. Aim #1 directly examines the effects of mechanical injury on
intercellular calcium signaling in astrocytes. Aim #2 examines the potential
mechanisms (i.e. gap junctions) involved in injury-induced alterations in
intercellular calcium signaling. Aim #3 examines potential therapeutic
interventions which could restore intercellular signaling. The results of the
proposed experiments may provide not only new insights into the pathophysiology
of TBI, but also potentially lead to the development of novel therapeutic
approaches for the treatment of the traumatically injured brain.
描述(由申请人提供):当前提案的目标是
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('CANDACE L. FLOYD', 18)}}的其他基金
Evaluation of 6SHG/EM1 as a treatment for spinal cord injury-induced neuropathic pain in a pig model
6SHG/EM1 治疗猪模型脊髓损伤引起的神经性疼痛的评价
- 批准号:
10237552 - 财政年份:2021
- 资助金额:
$ 4.64万 - 项目类别:
Evaluation of 6SHG/EM1 as a treatment for spinal cord injury-induced neuropathic pain in a pig model
6SHG/EM1 治疗猪模型脊髓损伤引起的神经性疼痛的评价
- 批准号:
10935563 - 财政年份:2021
- 资助金额:
$ 4.64万 - 项目类别:
Evaluation of 6SHG/EM1 as a treatment for spinal cord injury-induced neuropathic pain in a pig model
6SHG/EM1 治疗猪模型脊髓损伤引起的神经性疼痛的评价
- 批准号:
10512037 - 财政年份:2021
- 资助金额:
$ 4.64万 - 项目类别:
Role of dentate gyrus gating and neurogenesis in the pathophysiology of mild TBI
齿状回门控和神经发生在轻度 TBI 病理生理学中的作用
- 批准号:
9631191 - 财政年份:2018
- 资助金额:
$ 4.64万 - 项目类别:
Role of dentate gyrus gating and neurogenesis in the pathophysiology of mild TBI
齿状回门控和神经发生在轻度 TBI 病理生理学中的作用
- 批准号:
8370647 - 财政年份:2012
- 资助金额:
$ 4.64万 - 项目类别:
Role of dentate gyrus gating and neurogenesis in the pathophysiology of mild TBI
齿状回门控和神经发生在轻度 TBI 病理生理学中的作用
- 批准号:
8651954 - 财政年份:2012
- 资助金额:
$ 4.64万 - 项目类别:
Role of dentate gyrus gating and neurogenesis in the pathophysiology of mild TBI
齿状回门控和神经发生在轻度 TBI 病理生理学中的作用
- 批准号:
8481602 - 财政年份:2012
- 资助金额:
$ 4.64万 - 项目类别:
Neuroprotection selective Estrogen or Genistein in Spinal Cord Injury
脊髓损伤中选择性雌激素或金雀异黄酮的神经保护作用
- 批准号:
7373613 - 财政年份:2007
- 资助金额:
$ 4.64万 - 项目类别:
Neuroprotection selective Estrogen or Genistein in Spinal Cord Injury
脊髓损伤中选择性雌激素或金雀异黄酮的神经保护作用
- 批准号:
7209450 - 财政年份:2007
- 资助金额:
$ 4.64万 - 项目类别:
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