CORE--CELL PROCUREMENT AND PROCESSING RESOURCE

核心--电池采购及加工资源

• 批准号: 6668341
• 负责人: Shelly Heimfeld
• 金额: $ 25.65万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6668341
• 关键词: animal tissue; biomedical facility; cell population study; cooperative study; flow cytometry; hematopoietic stem cells; human subject; human tissue; tissue /cell culture

Cell procurement and processing resource. The Cell Procurement and processing Core will supply resources to help circumvent a critical limitation that impedes progress in the field of ex-vivo gene therapy, an inability to obtain sufficient qualities of purified stem cells or other cell populations needed for experimentation and optimization of the conditions required for efficient gene transduction. This Core will address that limitation by providing two specific functions. (1) The Core will make available to PEGT researchers as well as other NHLBI investigators large quantities of enriched stem cells or other specific blood cell populations for their in vitro studies, for primate model testing, and for pre-clinical trial usage. The Processing Core will obtain specimens form the Primate Core, and will operate a donor recruitment program, a cadaveric bone marrow program, and a Repository of Cryopreserved Research Specimens to supply the cell populations needed for large-scale cell selections. (2) Taking advantage of the instrumentation and expertise available from within the FHCRC Cryobiology Laboratory, the Core will provide clinical-grade cell processing and selections from both the human and primate components. The Core will also characterize these enriched and/or transduced cell populations by performing 2 and 3 color flow cytometric determinations, and quantitative CFC and LTC-IC assays. These diagnostic services will provide investigators with the ability to quality control results from the cell selections, and to determine efficiencies of transduction in the various stem/progenitor cell compartments. The ability of this Core to obtain, process, isolate and characterize large numbers of purified cells, and to deliver those cells to NHLBI researchers, will significantly enhanced studies in the areas of basic stem cell biology, and greatly facilitate the clinical advancement of novel gene therapy-based applications.

电池采购和加工资源。细胞获取和处理核心将提供资源，帮助克服阻碍离体基因治疗领域进展的关键限制，即无法获得足够质量的纯化干细胞或其他细胞群，用于实验和优化有效基因转导所需的条件。该核心将通过提供两个特定功能来解决这一限制。(1) Core将为PEGT研究人员以及其他NHLBI研究人员提供大量富集的干细胞或其他特定的血细胞群，用于他们的体外研究、灵长类动物模型测试和临床前试验。处理中心将从灵长类动物中心获取标本，并将开展供体招募计划、尸体骨髓计划和冷冻保存研究标本库，以提供大规模细胞选择所需的细胞群。(2)利用FHCRC低温生物学实验室现有的仪器和专业知识，Core将提供临床级细胞处理和人类和灵长类成分的选择。Core还将通过2色和3色流式细胞术测定以及定量CFC和LTC-IC测定来表征这些富集和/或转导的细胞群。这些诊断服务将为研究人员提供质量控制细胞选择结果的能力，并确定各种干细胞/祖细胞区室的转导效率。该核心能够获得、处理、分离和表征大量纯化细胞，并将这些细胞提供给NHLBI研究人员，这将显著增强基础干细胞生物学领域的研究，并极大地促进基于新型基因治疗应用的临床进展。