Core--Cellular therapy

细胞治疗共享资源是该联盟不可分割的一部分，在临床移植计划中发挥着关键作用。细胞疗法通过提供四种特定功能来帮助支持研究。首先，该资源提供了在分离采集、细胞处理、冷冻保存和细胞输注方面的关键仪器和技术专长。这一目标包括开发新的细胞选择和培养技术，以支持新的自体和同种异体治疗方案。其次，细胞疗法提供大规模处理能力，支持涉及人类、灵长类和犬类细胞的特定研究。这也包括开发新的细胞选择方法和临床前验证这种研究程序，以便在临床研究中实施。第三，实验室对临床和研究中使用的各种细胞成分进行定量的流式细胞术分析和体外集落形成分析。这些数据有助于确定哪些特定的细胞群可能与特定的临床结果相关。这反过来又有助于刺激新的移植工程方法和技术的发展，这些方法和技术可能会改善未来的移植研究。第四，细胞疗法获取并提供患者和正常捐赠者的样本，以支持研究项目。这些样本是通过以下方式获得的 IRB从新鲜标本和已建立的资料库获得批准的方案 用于研究研究的标本，将在帮助中心研究人员确定涉及植入、疾病控制、免疫重建和移植物抗宿主疾病的机制方面非常有用。这项申请要求继续支持为同行评议的研究发挥重要作用的资源。

The Cellular Therapy Shared Resource is an integral part of the Consortium and plays a critical role in the Clinical Transplantation program. Cellular Therapy helps support research by providing four specific functions. First, the resource provides the critical instrumentation and technical expertise in apheresis collection, cell processing, cryopreservation, and the infusion of cells. Included within this objective are the development of new cell selection and culturing techniques in support of novel autologous and allogeneic treatment protocols. Second, Cellular Therapy provides large-scale processing capabilities in support of specific research studies involving human, primate, and canine cells. This again encompasses the development of novel cell selection approaches and the preclinical validation of such research procedures for implementation in clinical studies. Third, the laboratory performs quantitative flow cytometry analyses and in vitro colony forming assays on various cellular components utilized for both clinical and research studies. These data are useful in determining which specific cell populations may be correlated with specific clinical outcomes. This in turn helps to stimulate the development of novel graft engineering approaches and techniques that could improve future transplantation studies. Fourth, Cellular Therapy procures and provides both patient and normal donor specimens in support of research projects. These samples, obtained under IRB approved protocols from fresh specimens and from an already established Repository Specimens for Research Studies, will be extremely useful in helping Center investigators to define the mechanisms involved in engraftment, disease control, immune reconstitution, and graft-versus-host disease. This application requests continued support for a resource which fulfills an essential role for peer reviewed research.