High Throughput Leukemia Diagnosis

描述（由申请人提供）：白血病复发性染色体易位是监测诊断、预后和治疗的有用细胞遗传学标记。传统的细胞遗传学研究不能在分子水平上识别所有的基因重排。此外，这是一个耗时和劳动密集的过程。我们已经开发了一种专利技术，可以同时进行高通量筛选和检测多种易位。这项名为ROCASH（特异性杂交后报告寡核苷酸捕获）的技术，利用标记的报告寡核苷酸在两步杂交过程中研究目标核酸。该方法的优点是提高了特异性和敏感性。最终用户不需要对样品进行标签。使用Luminex xMAP技术作为本程序的平台。它可以在一次检测中检测多达50个基因。在这项可行性研究中，将研究四种常见的复发易位。该项目的长期目标是开发一种用户友好的试剂盒，以筛选所有涉及复发性染色体易位的基因。该试剂盒将有助于白血病的诊断、分子分类、预后和治疗监测。

DESCRIPTION (provided by applicant): Recurrent chromosome translocations in leukemias are useful cytogenetic markers for monitoring diagnosis, prognosis and treatment. Traditional cytogenetic study cannot recognize all the gene rearrangements at the molecular level. Also, it is a time consuming and labor intensive procedure. We have developed a proprietary technology that will allow high throughput screening and detection of multiple translocations at a time. The technology, ROCASH (for Reporter Oligo Capturing After Specific Hybridization), utilizes a labeled reporter oligo to study target nucleic acid in a two-step hybridization procedure. The advantages of this method are increased specificity and sensitivity. The end user will not need to perform labeling of the samples. Luminex xMAP technology is used as a platform for this procedure. It can investigate up do 50 genes in one assay. In this feasibility study, four common recurrent translocations will be studied. The long-term goal of this proposed project is to develop a user-friendly reagent kit that will allow the screening of all the genes involved in recurrent chromosome translocations. The kit will be usefuL in diagnosis, molecular classification of the leukemias, prognosis, and treatment monitoring.