Affordable large population genomic DNA collections

描述（由申请人提供）：已知种族来源的大型人类基因组DNA集的可用性仍然是流行病学和疾病基因研究的一个持续问题。在这项资助中，两种宝贵的资源被结合起来解决DNA可用性问题。分子分期公司（MSI）开发了一种称为多重置换扩增（MDA）的技术，该技术能够产生106倍的完整人类基因组扩增，这些基因组忠实地代表原始模板，具有遗传基因座的出色代表性和高度的序列代表性。该技术将用于扩增一组广泛的已确定种族来源的存档人类基因组DNA样本，代表亚洲，非洲裔美国人和高加索人群，这些样本先前由Scripps研究所的Beutler博士在NIDDK资助的一个项目中收集。这些DNA样本将使用条形码控制的自动化过程进行扩增和排列，并将提供给其他NIH资助的研究人员。在SBIR的第一阶段，我们将建立扩增和阵列过程的自动化和质量控制。

DESCRIPTION (provided by applicant): The availability of large human genomic DNA sets of known ethnic origin remains a persistent problem for epidemiology and disease gene research. In this grant two valuable resources are combined to solve DNA availability problems. Molecular Staging Inc (MSI) has developed a technology, termed Multiple Displacement Amplification (MDA), that is capable of producing 106 fold amplification of whole human genomes that faithfully represent the original templates, with excellent representation of genetic loci and high fidelity of sequence representation. This technology will be used to amplify an extensive set of archived human genomic DNA samples of defined ethnic origin, representing Asian, African American and Caucasian populations, previously collected by Dr. Beutler of Scripps Research Institute during a project funded by NIDDK. These DNA samples will be amplified and arrayed using bar code controlled automated processes and will be provided to other NIH funded researchers. In Phase I of this SBIR we will set up automation and quality control of amplification and arraying processes.