ID-Tag Protein Profiling Array for the Molecular Charact

描述(由申请人提供)： 这项建议的长期目标是开发一种简单、可靠、敏感和成本效益高的技术平台，使从存档组织标本中提取的人类肿瘤样本中的多种抗原能够同时进行定量分析。在临床实验室环境中迫切需要这样一个平台来彻底确定癌症的特征。分子标记的多重分析可以补充和改进确定肿瘤起源的主要解剖位置、预测肿瘤行为和制定有效治疗方案的传统方法。超级阵列公司S专有的ID-Tag蛋白质图谱技术是一种基于抗体的多重检测技术。多个ID标签用于同时跟踪多个蛋白质目标。ID-Tag信号可进一步放大以提高检测下限。因此，开发一种简单的临床诊断工具是非常有希望的，它将是超敏感的，并允许同时分析来自档案材料的多种抗原，如石蜡包埋切片和酒精固定的细胞学标本。在这项第一阶段的提案中，SuperArray公司将与加州大学洛杉矶分校病理学系的Jian Yu Rao博士合作，评估ID-Tag蛋白质图谱阵列技术在人类癌症分子表征方面的可行性和应用。

DESCRIPTION (provided by applicant): The long-term objective of this proposal is to develop a simple, reliable, sensitive, and cost-effective technological platform that will allow the simultaneous and quantitative analysis of multiple antigens in human tumor samples derived from archived tissue specimens. Such a platform is urgently needed in the clinical laboratory setting to thoroughly characterize cancer. Multiplex analysis of molecular markers can augment and improve conventional methods for determining the primary anatomical site of tumor origin, predicting tumor behavior, and formulating effective therapy. SuperArray Inc.'s proprietary ID-Tag Protein Profiling technology is an antibody-based multiplex detection. Multiple ID tags are used to track multiple protein targets simultaneously. ID-tag signal can be further amplified to increase assay detection limit. Therefore, it is very promise to develop a simple clinical diagnostic tool that will be supersensitive and allow the simultaneous analysis of multiple antigens from archival material such as paraffin-embedded sections and alcohol-fixed cytology specimens. In this phase I proposal, SuperArray, Inc., will collaborate with Dr. Jian Yu Rao, Department of Pathology, University of California at Los Angeles, to evaluate the feasibility and application of the ID-Tag Protein Profiling Array technology for the molecular characterization of human cancers.