Mechanisms of Nucleic acid Uptake by Renal Epithelium

肾上皮摄取核酸的机制

• 批准号: 6616173
• 负责人: PAUL Evan KLOTMAN
• 金额: $ 32.84万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-20 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6616173
• 关键词: DNA; biological transport; epithelium; gene targeting; genetically modified animals; high performance liquid chromatography; kidney; laboratory rat; malate dehydrogenase; mass spectrometry; matrix assisted laser desorption ionization; membrane channels; nucleic acids; polymerase chain reaction; protein protein interaction; protein structure function; tissue /cell culture

DESCRIPTION (provided by applicant): One of the opportunities to intervene in a disease process is to identify the molecules that participate and then inhibit them. This can be done in theory by identifying the nucleic acids that encode the protein and using a complementary strand of DNA to block this from happening. Unfortunately, while the concept works in solutions of fluid, the ability to do this in a cell has been more difficult. One of the major problems has been understanding how these strands of DNA get into cells. The purpose of this project is to explore this issue. We have previously shown that single stranded DNA can move through a highly specific channel. We purified this protein complex and have been identifying what each component of this complex does. We have now identified one of the subunits of this channel as a common enzyme that serves also to regulate DNA uptake. The current proposal will evaluate other subunits and participants in this activity. We hope to use this information to design DNA molecules as therapeutics to treat disease.

描述（由申请人提供）： 干预疾病过程的机会之一是识别 参与并抑制它们的分子这在理论上可以通过 鉴定编码所述蛋白质的核酸，并使用互补的 DNA链来阻止这种情况的发生不幸的是，虽然这个概念 在液体溶液中起作用，在细胞中这样做的能力已经越来越多， 难其中一个主要的问题是理解这些链是如何 DNA进入细胞。本项目的目的就是探讨这一问题。我们 先前已经证明，单链DNA可以通过一个高度 具体频道我们纯化了这种蛋白质复合物， 这个复合体的每一个组成部分是做什么的。我们现在已经确定了一个 亚基作为一种共同的酶，也用于调节DNA 摄取。目前的建议将评估其他分单位和参与者， 这个活动。我们希望利用这些信息来设计DNA分子， 治疗疾病的药物