Development of a novel optical system for measuring kinetic parameters of synthetic lectin systems

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Measuring accurate association/dissociation rates of molecules with anomalously high binding affinities proves to be incredibly difficult due to the speed at which the association/disassociation takes place (Piran 1990). Optical methods have the advantage of typically being much faster than typical chemical-based techniques (i.e. ITC), along with allowing bulk determination rather than surface bound. Using a novel technique known as backscattering interferometry (BSI) (Bornhop 2007), I am working towards determining the kinetic parameters of previously unmeasured molecule pairs, as well as improving on the current state of BSI. In addition to this, I am working towards creating an novel interpretation of interferometric scattering (iSCAT) microscopy that will be able to view binding events in real time (Young 2018).