PDZ Interaction and Inward Rectifier K+ Channel Function

PDZ 交互和内向整流 K 通道功能

• 批准号: 6623065
• 负责人: CAROL A VANDENBERG
• 金额: $ 28.03万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6623065
• 关键词: affinity chromatography; cell line; chimeric proteins; glycosylation; immunoprecipitation; laboratory mouse; membrane potentials; membrane proteins; nerve /myelin protein; phosphorylation; potassium channel; protein binding; protein degradation; protein kinase A; protein protein interaction; protein sequence; protein structure function; tissue /cell culture; transcription factor; transfection; yeast two hybrid system

DESCRIPTION (provided by applicant): The strong inward rectifier potassium channels are involved in action potential repolarization, modulation of cell excitability, and determination of cell resting potential in the brain and other excitable cells. Indeed, mutations in these channels are associated with defects in cell excitability, periodic paralysis, cardiac arrhythmias, and developmental abnormalities. Recently, it has been demonstrated that proteins that are associated with ion channels may modulate the functional properties of the channels, and such interactions may have profound implications in normal physiology and disease. Defects in channel-interacting proteins could lead to problems in channel expression, membrane targeting, association of channels with signaling complexes, and channel electrical activity. This proposal aims to identify and characterize the proteins that interact with inward rectifier potassium channels. Recently, we have identified such an interaction between inward rectifier channels and the scaffolding protein SAP97 in the brain. The goal of this project is to identify the complex of proteins that associate with inward rectifier potassium channels and to analyze the functional ability of these proteins to cluster, localize and regulate the activity of the channels.

描述（由申请人提供）： 强内向整流钾通道参与动作电位 复极化，细胞兴奋性的调节，以及细胞 大脑和其他易兴奋细胞的静息电位事实上， 这些通道与细胞兴奋性、周期性 瘫痪心律失常和发育异常近日 已经证明与离子通道相关的蛋白质可以 调节通道的功能特性，并且这种相互作用可以 对正常生理和疾病有着深远的影响。缺陷 通道相互作用蛋白可能导致通道表达的问题， 膜靶向，通道与信号传导复合物的结合，以及 通道电活动。 该提案旨在识别和表征与蛋白质相互作用的蛋白质。 内向整流钾通道最近，我们发现了这样一种 内向整流通道与支架蛋白SAP97的相互作用 在大脑中。这个项目的目标是识别蛋白质的复合物 与内向整流钾通道相关的蛋白质， 这些蛋白质聚集、定位和调节蛋白质的功能能力， 渠道的活动。