PDZ Interaction and Inward Rectifier K+ Channel Function
PDZ Interaction and Inward Rectifier K+ Channel Function
批准号:
6876700
负责人:
CAROL A VANDENBERG
金额:
$28.03万
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-04-01 至 2007-03-31
关键词:
affinity chromatographycell linechimeric proteinsglycosylationimmunoprecipitationlaboratory mousemembrane potentialsmembrane proteinsnerve /myelin proteinphosphorylationpotassium channelprotein bindingprotein degradationprotein kinase Aprotein protein interactionprotein sequenceprotein structure functiontissue /cell culturetranscription factortransfectionyeast two hybrid system
中文摘要
描述(由申请人提供):
强内向整流钾通道参与动作电位
复极化、细胞兴奋性的调节以及细胞的测定
大脑和其他可兴奋细胞的静息电位。事实上,突变
这些通道与细胞兴奋性、周期性缺陷有关
瘫痪、心律失常和发育异常。最近,它
已证明与离子通道相关的蛋白质可能
调节通道的功能特性,这种相互作用可能
对正常生理和疾病具有深远的影响。缺陷于
通道相互作用蛋白可能会导致通道表达问题,
膜靶向、通道与信号复合物的关联,以及
通道电活动。
该提案旨在识别和表征与相互作用的蛋白质
内向整流钾通道。最近,我们发现了这样一个
内向整流通道与支架蛋白 SAP97 之间的相互作用
在大脑中。该项目的目标是鉴定蛋白质复合物
与内向整流钾通道相关并分析
这些蛋白质聚集、定位和调节的功能能力
渠道的活动。
英文摘要
DESCRIPTION (provided by applicant):
The strong inward rectifier potassium channels are involved in action potential
repolarization, modulation of cell excitability, and determination of cell
resting potential in the brain and other excitable cells. Indeed, mutations in
these channels are associated with defects in cell excitability, periodic
paralysis, cardiac arrhythmias, and developmental abnormalities. Recently, it
has been demonstrated that proteins that are associated with ion channels may
modulate the functional properties of the channels, and such interactions may
have profound implications in normal physiology and disease. Defects in
channel-interacting proteins could lead to problems in channel expression,
membrane targeting, association of channels with signaling complexes, and
channel electrical activity.
This proposal aims to identify and characterize the proteins that interact with
inward rectifier potassium channels. Recently, we have identified such an
interaction between inward rectifier channels and the scaffolding protein SAP97
in the brain. The goal of this project is to identify the complex of proteins
that associate with inward rectifier potassium channels and to analyze the
functional ability of these proteins to cluster, localize and regulate the
activity of the channels.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
IDENTIFICATION OF PROTEINS ASSOCIATED WITH INWARD RECTIFIER POTASSIUM CHANNELS
-
批准号:7182427
-
项目类别:
-
资助金额:$0.4万
-
财政年份:2005
-
负责人:CAROL A VANDENBERG
-
依托单位:
INWARD RECTIFIER POTASSIUM CHANNEL ASSOCIATED PROTEINS
-
批准号:6979708
-
项目类别:
-
资助金额:$0.36万
-
财政年份:2004
-
负责人:CAROL A VANDENBERG
-
依托单位:
PDZ Interaction and Inward Rectifier K+ Channel Function
-
批准号:6460895
-
项目类别:
-
资助金额:$30.33万
-
财政年份:2002
-
负责人:CAROL A VANDENBERG
-
依托单位:
PDZ Interaction and Inward Rectifier K+ Channel Function
-
批准号:6623065
-
项目类别:
-
资助金额:$28.03万
-
财政年份:2002
-
负责人:CAROL A VANDENBERG
-
依托单位:
PDZ Interaction and Inward Rectifier K+ Channel Function
-
批准号:6721322
-
项目类别:
-
资助金额:$28.03万
-
财政年份:2002
-
负责人:CAROL A VANDENBERG
-
依托单位:
MOLECULAR PHYSIOLOGY OF CARDIAC POTASSIUM CHANNELS
-
批准号:2838948
-
项目类别:
-
资助金额:$21.26万
-
财政年份:1989
-
负责人:CAROL A VANDENBERG
-
依托单位:
PHYSIOLOGY OF CARDIAC ION CHANNELS
-
批准号:3472420
-
项目类别:
-
资助金额:$10.02万
-
财政年份:1989
-
负责人:CAROL A VANDENBERG
-
依托单位:
PHYSIOLOGY OF CARDIAC ION CHANNELS
-
批准号:2220128
-
项目类别:
-
资助金额:$10.29万
-
财政年份:1989
-
负责人:CAROL A VANDENBERG
-
依托单位:
MOLECULAR PHYSIOLOGY OF CARDIAC POTASSIUM CHANNELS
-
批准号:2028425
-
项目类别:
-
资助金额:$19.66万
-
财政年份:1989
-
负责人:CAROL A VANDENBERG
-
依托单位:
PHYSIOLOGY OF CARDIAC ION CHANNELS
-
批准号:3472418
-
项目类别:
-
资助金额:$8.33万
-
财政年份:1989
-
负责人:CAROL A VANDENBERG
-
依托单位:
PHYSIOLOGY OF CARDIAC ION CHANNELS
-
批准号:3472419
-
项目类别:
-
资助金额:$9.42万
-
财政年份:1989
-
负责人:CAROL A VANDENBERG
-
依托单位:
MOLECULAR PHYSIOLOGY OF CARDIAC POTASSIUM CHANNELS
-
批准号:6125770
-
项目类别:
-
资助金额:$22.11万
-
财政年份:1989
-
负责人:CAROL A VANDENBERG
-
依托单位:
MOLECULAR PHYSIOLOGY OF CARDIAC POTASSIUM CHANNELS
-
批准号:2609269
-
项目类别:
-
资助金额:$20.44万
-
财政年份:1989
-
负责人:CAROL A VANDENBERG
-
依托单位:
PHYSIOLOGY OF CARDIAC ION CHANNELS
-
批准号:3472421
-
项目类别:
-
资助金额:$10.95万
-
财政年份:1989
-
负责人:CAROL A VANDENBERG
-
依托单位:
MOLECULAR PHYSIOLOGY OF CARDIAC POTASSIUM CHANNELS
-
批准号:2220130
-
项目类别:
-
资助金额:$22.94万
-
财政年份:1989
-
负责人:CAROL A VANDENBERG
-
依托单位:
CHANNEL PROPERTIES--RECONST'D VESICLES & CULTURED CELLS
-
批准号:3053561
-
项目类别:
-
资助金额:$2.0万
-
财政年份:1984
-
负责人:CAROL A VANDENBERG
-
依托单位:
海外基金