TECHNIQUES FOR MODELING HUMAN CANCER IN MICE

目前迫切需要具有精确、可重复的人类癌症小鼠模型来推进对人类癌症的病因、治疗和预防的研究。为了充分利用这些模型，调查必须是多学科的，利用不同领域研究人员的专业知识。然而，对小鼠生物学和病理学的理解是必不可少的，使用其他模型系统或在其他研究学科受过训练的研究人员也带来了同样重要的见解。为了实现对癌症控制和预防的跨学科、转化性研究，必须为年轻的研究人员提供机会，让他们在准备和分析人类癌症小鼠模型的技术方面进行实践培训。为了满足这一需求，杰克逊实验室（TJL）将提供一个关于特定人类癌症小鼠模型的年度系列研讨会；第一个将重点放在乳腺癌的小鼠模型上。后续研讨会的主题将在与NCI人类癌症小鼠模型联盟指导委员会、TJL教师和其他癌症研究专家协商后确定。教师将从TJL员工和特邀专家中挑选；课程将包括讲座和实践培训。参与者将处于博士后后期/早期独立研究员水平，并将通过竞争性选拔。第一次讲习班的具体目标是：1)展示繁殖和维持高健康水平遗传定义突变菌株所需的基本畜牧业技术；2)演示在正常生殖周期和癌变过程中分析乳腺解剖的技术；3)演示体内和体外乳腺生长控制技术；4)引入分析乳腺功能和肿瘤进展的新技术。

Mouse models with heritable cancers that are accurate, reproducible models of human cancers are urgently needed to advance research into the etiology, treatment, and prevention of human cancers. To exploit these models fully, investigations must be multi-disciplinary, drawing on the expertise of researchers in diverse fields. Understanding of mouse biology and pathology are essential, however, investigators using other model systems or who were trained in other research disciplines bring equally important insights. To achieve a cross-disciplinary, translational research focus on cancer control and prevention, opportunities must be provided for young investigators to have hands-on training in the techniques for preparing and analyzing mouse models of human cancers. To address this need The Jackson Laboratory (TJL) will offer an annual workshop series on mouse models of specific human cancers; the first will focus on mouse models for mammary cancer. The topics of subsequent workshops will be determined after consultation with the NCI Mouse Models of Human Cancers Consortium Steering Committee, TJL faculty, and other experts in cancer research. Faculty will be drawn from TJL staff and invited experts; sessions will include both lectures and hands-on training. Participants will be at the late-postdoctoral/early independent researcher level and will be competitively selected. The specific objectives of the first workshop are to: 1) demonstrate basic animal husbandry techniques needed to propagate and maintain genetically-defined mutant strains at high health levels; 2) demonstrate techniques for analyzing mammary gland anatomy throughout the normal reproductive cycle and during carcinogenesis; 3) demonstrate techniques for in vivo and in vitro manipulation of mammary gland growth; 4) introduce novel technologies for analyzing mammary gland function and neoplastic progression.