Antigenic variation of Anaplasma marginale

边缘无形体的抗原变异

• 批准号: 6560747
• 负责人: PATRICK F MEEUS
• 金额: $ 11.78万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6560747
• 关键词: Rickettsiales; bacterial antigens; bacterial genetics; cow; genetic polymorphism; host organism interaction; microorganism immunology; polymerase chain reaction; ticks; tissue /cell culture

DESCRIPTION (provided by applicant): Research proposal: The pathogenesis of ehrlichial and related tick-borne rickettsial pathogens of humans and animals is directly linked to their ability to evade the immune system. Antigenic variation of surface proteins is a major evasion strategy common to all ehrlichial pathogens, often involving very similar antigens. Anaplasma marginale, a bovine pathogen, is closely related to Anaplasma (Ehrlichia) phagocytophila, the causative agent of human granulocytic ehrlichiosis. Investigations of bovine anaplasmosis offer a unique opportunity to study the mechanisms of antigenic variation in a natural animal model under controlled conditions. This project will examine the mechanisms and extent of antigenic variation of MSP3, the major antigen recognized by the hosts immune system, and related antigens. It will also investigate the influences of different environments: the mammalian host, the tick vector and in-vitro cultures, on the expression of the MSP3 antigen in a clonal line of A. marginale. The candidate: The principal investigator is a veterinarian with extensive field experience in parasitic and tick-borne diseases and with a strong desire to become an independent researcher in this field. In preparation for this, the candidate has enrolled in an infectious disease Ph.D. program and has completed courses in statistics, molecular biology, immunology and pathogenesis and has been admitted to candidacy. This proposal will allow his development as an independent researcher and will help elucidating pathogenic mechanisms of vector-borne pathogens in animals and humans. The investigator hopes to apply these findings and the expertise gained toward the development of innovative control strategies. Environment: The training will occur in an interactive environment of clinical and basic scientists. The mentors have extensive experience in the pathogenesis of and immune responses to vector-borne pathogens and have successfully trained previous candidates. The program provides an excellent framework for the proposed training.

描述（由申请人提供）：研究方案：人类和动物的埃立克体和相关蜱传立克次体病原体的发病机制与其逃避免疫系统的能力直接相关。表面蛋白的抗原变异是所有埃立克体病原体共同的主要逃避策略，通常涉及非常相似的抗原。边缘无浆体是一种牛病原体，与人粒细胞埃立克体病的病原体--嗜吞噬细胞无浆体（埃立克体）密切相关。牛无形体病的调查提供了一个独特的机会，研究抗原变异的机制，在自然动物模型在受控条件下。本研究将探讨宿主免疫系统识别的主要抗原MSP 3及其相关抗原的抗原变异机制和程度。它还将研究不同环境的影响：哺乳动物宿主，蜱载体和体外培养物，对MSP 3抗原在A.边缘。 候选人：主要研究者是一名兽医，在寄生虫和蜱传疾病方面具有丰富的现场经验，并强烈希望成为该领域的独立研究人员。为了准备这一点，候选人已经报名参加了传染病博士学位。 计划，并已完成了统计学，分子生物学，免疫学和发病机制的课程，并已被录取的候选人资格。这一提议将使他能够发展成为一名独立的研究人员，并将有助于阐明动物和人类媒介传播病原体的致病机制。研究人员希望将这些发现和获得的专业知识应用于创新控制策略的开发。 环境：培训将在临床和基础科学家的互动环境中进行。导师在病媒传播病原体的发病机制和免疫反应方面具有丰富的经验，并成功地培训了以前的候选人。该计划为拟议的培训提供了一个很好的框架。