ROLE OF STRESS IN EXPRESSION OF MAMMALIAN EICOSANOIDS IN RED ALGAE

描述（根据应用改编）：本提案的总体目标 是检验类花生酸化合物在藻类中的表达 与压力条件有关。假设这种关系是基于 类花生酸作为细胞内信号的作用。类花生酸在生物学上 参与许多基本人体代谢功能的活性分子， 在哺乳动物体内平衡中发挥重要作用， 几乎每一个哺乳动物的组织和体液， 生理活动的范围。建立类花生酸进化为 压力管理化合物，它们的作用已经通过 进化将提供一种功能同源性，其主要作用之一是， 动物，其中脂氧合酶介导的白三烯和脂氧素的形成 作为一个信号，启动生物体内的应激反应。 这些目标将通过培养目标物种Murrayella来实现 periclados，并检查脂氧合酶诱导的时间过程， 类花生酸表达和随后的应激蛋白表达。应激蛋白 鉴定将采用二维SDS PAGE。类花生酸浓度 将使用ELISA测定来测量。Murrayella的分子探针 将开发脂氧合酶，并将得到的基因或基因片段 用于产生多克隆抗体。这些抗体将被用于 在应激条件下使用ELISA跟踪脂氧合酶诱导 测定。类花生酸作为一种信号，将用Murrayella进行测试， 已经被培养成缺乏脂氧合酶产物。

Description (Adapted from Application): The overall objective of this proposal is to examine the hypothesis that expression of eicosanoid compounds in algae is related to stress conditions. The relationship is hypothesized to be based on eicosanoid action as an intracellular signal. Eicosanoids are biologically active molecules that are involved in many basic human metabolic functions, playing important roles in mammalian homeostasis where they are present in virtually every mammalian tissue and body fluid and where they exert a broad spectrum of physiological activities. Establishment that eicosanoids evolved as stress management compounds, and that their role has been conserved through evolution would provide a functional homology with one of its major roles in animals, where the lipoxygenase-mediated formation of leukotrienes and lipoxins serves as a signal to initiate stress responses within the organisms. The objectives will be carried out by culturing the target species, Murrayella periclados, and examining the time courses of lipoxygenase enzyme induction, eicosanoid expression, and subsequent stress protein expression. Stress protein identification will employ two-dimensional SDS PAGE. Eicosanoid concentrations will be measured using ELISA assays. A molecular probe for Murrayella lipoxygenase will be developed and the resultant gene or gene fragment will be utilized to produce polyclonal antibodies. The antibodies will then be used to follow lipoxygenase induction following stress conditions utilizing ELISA assays. That eicosanoids act as a signal will be tested with Murrayella that has been cultured to be deficient in lipoxygenase products.