Studies on Cryptosporidium parvum Type 1

说明（申请人提供）：这是一份修订后的提案 （R 01 AI 150471 -01）对PA 99-124的响应。该项目的目标是 隐孢子虫1型的综合特征，最 普通人群和人类隐孢子虫病的常见病原体 艾滋病因为直到最近1C型。小孢子不能在 在实验室中，很少对这一亚组进行研究， 表征了因此，小牛繁殖型的表型特性 2个卵囊外推至整个物种。鉴于重大的 1型隐孢子虫病引起的公共卫生重要性。帕尔武姆 该建议重点是两个方面：1）广泛表征C. parvum 1型的基因型和表型特性，以及其寿命 循环和与寄主的相互作用;（2）比较C.小孢子1型， 类型2，目的是为了更好地了解物种C。parvum 以及这种寄生虫引起的疾病最近拟定的 一种适合1型和2型猪繁殖的无菌猪模型， 促进了几种1型分离株的研究和 标准化C.来自同一宿主的两种类型的细小病毒分离物。 混合感染中1型和2型分离株的相互作用也将被 在猪模型中研究。本研究中包括的1型分离株将来自 从慢性和急性感染以及从不同的地理区域。的 本研究包括三个具体目标：第一个目标是全面分析 10个1C型。小孢子分离物在第二个目标中， 将使用猪模型比较1型和2型分离株的相互作用 和细胞培养模型。第三个目的是研究遗传的程度， 两种类型C.小到 确定它们是否属于一个或两个物种。

DESCRIPTION (Provided by the applicant): This is a revised proposal (R01AI150471-01) in response to PA 99-124. The goal of this project is a comprehensive characterization of Cryptosporidium parvum type 1, the most common agent of human cryptosporidiosis in the general population and in people with AIDS. Because until recently type 1 C. parvum could not be propagated in the laboratory, this subgroup is rarely studied and remains poorly characterized. As a consequence, phenotypic properties of calf-propagated type 2 oocysts are extrapolated to the whole species. In light of the significant public health importance of cryptosporidiosis caused by type 1 C. parvum, the emphasis of this proposal is twofold: 1) to extensively characterize C. parvum type 1 with respect to genotypic and phenotypic properties, as well as its life cycle and interaction with the host, and (2) to compare C. parvum type 1 and type 2 with the aim of gaining a better understanding of the species C. parvum and the disease caused by this parasite. The recent development of a gnotobiotic pig model suitable for propagation of type 1 and type 2 will facilitate the study of several type 1 isolates and the comparison of standardized C. parvum isolates of both types originating from the same host. The interaction of type 1 and type 2 isolates in mixed infections will also be studied in the pig model. Type 1 isolates included in this study will originate from chronic and acute infections and from different geographical areas. The study includes three specific aims: the first aim is a comprehensive analysis of ten type 1 C. parvum isolates. In the second aim the host-parasite interaction of type 1 and type 2 isolates will be compared using the pig model and cell culture models. The third aim investigates the extent of genetic variation and exchange within and between the two types of C. parvum to determine whether they belong to one or two species.