How does cyclic-di-GMP regulate biosynthesis of a key P. syringae phytotoxin?

环二 GMP 如何调节关键的丁香假单胞菌植物毒素的生物合成？

• 批准号: 2237746
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2237746
• 关键词: How; does; cyclic; di; GMP

Pseudomonas syringae is a widespread plant pathogen responsible for yield losses in crops worldwide. The phytotoxin coronatine (COR) is produced by several P. syringae pathovars and plays an important role in initiating plant infections. COR suppresses plant immunity, inhibits the closure of stomata, and induces chlorosis (yellowing) of infected tissues. We recently showed that the widespread bacterial signalling molecule cyclic-di-GMP (cdG) binds to three enzymes of the COR biosynthesis pathway. This finding implicates cdG in COR regulation and in the control of P. syringae plant infection. In this project, we will use a combination of genetics, biochemistry and structural biology to define how cdG-binding controls COR biosynthesis in P. syringae. The project will first conduct an indepth biochemical analysis of cdG-binding to the COR-biosynthetic enzymes. The relationship between cdG and regulation of each enzyme will be further refined through structural characterization by X-ray crystallography. In parallel, we will use genetics, molecular biology and plant infection experiments to determine the physiological relevance of cdG-binding to COR biosynthesis, and its impact on P. syringae virulence in planta.

丁香假单胞菌是一种广泛存在的植物病原体，在世界范围内造成作物产量损失。植物毒素coronatine （COR）由几种紫丁香属病原菌产生，在引发植物感染中起重要作用。COR抑制植物免疫，抑制气孔关闭，诱导感染组织的黄化。我们最近发现广泛存在的细菌信号分子环二gmp （cdG）与COR生物合成途径的三种酶结合。这一发现提示cdG参与了COR的调控和丁香属植物侵染的控制。在本项目中，我们将结合遗传学、生物化学和结构生物学来确定cdg结合如何控制丁香属植物COR的生物合成。该项目将首先对cdg与co生物合成酶的结合进行深入的生化分析。cdG与每种酶的调节之间的关系将通过x射线晶体学的结构表征进一步细化。同时，我们将利用遗传学、分子生物学和植物侵染实验来确定cdg结合与COR生物合成的生理相关性及其对丁香假单胞菌（P. syringae）植物毒力的影响。