Improving microRNA target prediction going beyond the seed

改进 microRNA 目标预测，超越种子范围

• 批准号: 2243600
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2243600
• 关键词: Improving; microRNA; target; prediction; going

MicroRNAs (miRNAs) are a class of non-coding RNAs ~22nt in length known to play a pivotal role in the regulation of gene expression. They bind to their targets by Watson-Crick base pairing, leading to translational inhibition, deadenylation, and eventual mRNA degradation. Binding usually occurs in the 5' portion of the miRNA between positions 2-7 known as the miRNA 'seed' sequence. Different miRNAs can share identical seed sequences, and therefore computational prediction methods will currently report identical targets for all miRNAs from the same family. However, recent studies have shown that additional binding outside of the seed region can confer specificity of targeting to different miRNAs sharing a similar or identical seed. This studentship will investigate the effect of supplementary (non-seed) binding on the efficacy and specificity of miRNA-mediated repression using miRNA over-expression/repression RNA-Seq datasets. These will be used to precisely characterise the position(s) of supplementary binding sites and to compare the effectiveness and specificity of targeting against seed-only sites across a broad range of miRNAs. Results from these experiments will then be incorporated into our FilTar tool for computational miRNA target prediction.

MicroRNA（miRNAs）是一类长度约为22 nt的非编码RNA，在基因表达调控中起着重要作用。它们通过Watson-Crick碱基配对与其靶点结合，导致翻译抑制、去腺苷化和最终mRNA降解。结合通常发生在称为miRNA“种子”序列的位置2-7之间的miRNA的5'部分中。不同的miRNA可以共享相同的种子序列，因此计算预测方法目前将报告来自同一家族的所有miRNA的相同靶标。然而，最近的研究表明，种子区域外的额外结合可以赋予靶向共享相似或相同种子的不同miRNA的特异性。 本研究将使用miRNA过表达/抑制RNA-Seq数据集研究补充（非种子）结合对miRNA介导的抑制的有效性和特异性的影响。这些将用于精确定位补充结合位点的位置，并在广泛的miRNA中比较靶向仅种子位点的有效性和特异性。这些实验的结果将被整合到我们的FilTar工具中，用于计算miRNA靶点预测。