Heterotrimeric G Protein Signaling in Arabidopsis

拟南芥中的异三聚体 G 蛋白信号转导

• 批准号: 6513643
• 负责人: ALAN M. JONES
• 金额: $ 31.56万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6513643
• 关键词: Arabidopsis; G protein; biological signal transduction; cell proliferation; confocal scanning microscopy; gene expression; guanine nucleotide binding protein; hormone receptor; indoleacetate; phytohormones; plant growth /development; plant growth regulators; polymerase chain reaction; protein protein interaction; protein structure function; receptor coupling; root; seeds

DESCRIPTION (provided by applicant): In most metazoans, different heterotrimeric G protein complexes couple many different upstream signals to different downstream effectors such as adenyl cyclase, ion channels, and phospholipases. However, in Arabidopsis, there is only a single canonical heterotrimeric G protein complex that nonetheless controls different plant responses. We do not know how this is accomplished nor do we know what receptors activate this G protein complex. Recent technical advances from my group using both gain- and loss-of-function approaches to understand the function of Arabidopsis G protein subunits will enable us to determine the molecular role of this G protein complex in various signaling pathways. There are three focus areas for this project: auxin control of root development, hypocotyl development; and multi-signal control of seed germination. Specifically, we will: 1. determine auxin responsiveness of G protein mutants and overexpressors and examine the signal cross-talk controlling seed germination, 2. identify and characterize genetic and physical interactors of Galpha and Gbeta using extragenic suppressor screens including activation mutagenesis and using cytosolic protein-protein interaction assays, and 3. test putative functions of a small set of candidate GPCRs using reverse genetic approaches. In summary, the goal is to test the hypothesis that auxin utilizes heterotrimeric G proteins in signal transduction. This work also addresses a longstanding problem on the mechanism of signal cross-talk. Because, the single Arabidopsis heterotrimeric G protein appears to be central to multiple signal pathways, genetic studies using Arabidopsis may reveal the core mechanism of signal integration, which is likely to be shared universally.

描述（由申请人提供）：在大多数后生动物中，情况不同