Heterotrimeric G Protein Signaling in Arabidopsis
Heterotrimeric G Protein Signaling in Arabidopsis
批准号:
6942956
负责人:
ALAN M. JONES
金额:
$28.56万
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-09-01 至 2008-08-31
关键词:
ArabidopsisG proteinbiological signal transductioncell proliferationconfocal scanning microscopygene expressionguanine nucleotide binding proteinhormone receptorindoleacetatephytohormonesplant growth /developmentplant growth regulatorspolymerase chain reactionprotein protein interactionprotein structure functionreceptor couplingrootseeds
中文摘要
描述(由申请人提供):在大多数后生动物中,不同
异三聚体G蛋白复合体将许多不同的上游信号偶联到
不同的下游效应因子,如腺苷环化酶、离子通道和
磷脂酶。然而,在拟南芥中,只有一个典型的
控制不同植物的异三聚体G蛋白复合体
回应。我们不知道这是如何实现的,也不知道是什么
受体激活这种G蛋白复合体。我的最新技术进步
小组使用增益和损失函数的方法来理解
拟南芥G蛋白亚基的功能将使我们能够确定
这种G蛋白复合体在各种信号通路中的分子作用。那里
是该项目的三个重点领域:根发育的生长素控制,
下胚轴发育;种子萌发的多信号调控。
具体地说,我们将:1.确定G蛋白突变体的生长素反应性
和过表达因子,并检测信号串扰控制种子
萌发,2.确定和表征遗传和物理交互作用
Galpha和Gbeta使用包括激活在内的基因外抑制物筛选
诱变和利用胞浆蛋白-蛋白相互作用分析,以及3.测试
基于反向遗传的一小部分候选GPCR的推测功能
接近了。总而言之,我们的目标是检验生长素利用
异三聚体G蛋白在信号转导中的作用这项工作还解决了一个
关于信号串扰机制的长期问题。因为,单曲
拟南芥异源三聚体G蛋白似乎是多种信号的中心
利用拟南芥的途径、遗传学研究可能揭示
信号集成,这很可能是普遍共享的。
英文摘要
DESCRIPTION (provided by applicant): In most metazoans, different
heterotrimeric G protein complexes couple many different upstream signals to
different downstream effectors such as adenyl cyclase, ion channels, and
phospholipases. However, in Arabidopsis, there is only a single canonical
heterotrimeric G protein complex that nonetheless controls different plant
responses. We do not know how this is accomplished nor do we know what
receptors activate this G protein complex. Recent technical advances from my
group using both gain- and loss-of-function approaches to understand the
function of Arabidopsis G protein subunits will enable us to determine the
molecular role of this G protein complex in various signaling pathways. There
are three focus areas for this project: auxin control of root development,
hypocotyl development; and multi-signal control of seed germination.
Specifically, we will: 1. determine auxin responsiveness of G protein mutants
and overexpressors and examine the signal cross-talk controlling seed
germination, 2. identify and characterize genetic and physical interactors of
Galpha and Gbeta using extragenic suppressor screens including activation
mutagenesis and using cytosolic protein-protein interaction assays, and 3. test
putative functions of a small set of candidate GPCRs using reverse genetic
approaches. In summary, the goal is to test the hypothesis that auxin utilizes
heterotrimeric G proteins in signal transduction. This work also addresses a
longstanding problem on the mechanism of signal cross-talk. Because, the single
Arabidopsis heterotrimeric G protein appears to be central to multiple signal
pathways, genetic studies using Arabidopsis may reveal the core mechanism of
signal integration, which is likely to be shared universally.
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海外基金