Regulation of Prostaglandins in Periovulatory Follicles
排卵周围卵泡中前列腺素的调节
基本信息
- 批准号:6620850
- 负责人:
- 金额:$ 25.04万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-02-01 至 2006-01-31
- 项目状态:已结题
- 来源:
- 关键词:cell cell interaction corpus luteum cow eicosanoid metabolism fatty acid biosynthesis follicle stimulating hormone graafian follicles granulosa cell hormone regulation /control mechanism in situ hybridization luteinizing hormone messenger RNA ovulation oxytocin progesterone progesterone receptors prostaglandin E prostaglandin endoperoxide synthase prostaglandin receptor prostaglandins radioimmunoassay receptor expression reproductive development tissue /cell culture
项目摘要
DESCRIPTION (provided by applicant): The LH/FSH surge sets in motion a cascade
of events that culminates in the remarkable rupture of the follicular wall at
the apex and the subsequent release of the oocyte-cumulus complex. Despite its
critical importance to reproduction and fertility, and despite decades of
research, there are many gaps in our knowledge of the regulation of the
ovulatory process. Many regulators and pathways have been hypothesized to be
involved in mammalian ovulation, but results of previous studies have
highlighted essential roles for progesterone and prostaglandins (PGs). Cattle
provide an ideal animal model for studying the complex events of the
periovulatory period. We have validated a model for inducing periovulatory
follicular development in cattle and we now propose to use it to test
hypotheses about these two key regulators of ovulation and their receptors. In
Specific Aims 1 and 2, the timing, cellular site(s) of synthesis, biosynthetic
pathway, and hormonal regulation of the periovulatory increase in PG synthesis
will be examined. The hypothesis to be tested is that both theca and granulosa
cells respond to the preovulatory LH/FSH surge with an increase in PG
production, mediated by the surge-induced rise in progesterone, which in turn
induces PGF2a synthesis, via reduction from PGE2. In Specific Aim 3 we will
test the hypothesis that the effects of progesterone on prostaglandin
production by the periovulatory follicle are at least partially mediated
through progesterone-induced increases in oxytocin. Although PGs are essential
for ovulation, virtually nothing is known about the expression of PG receptors
during the periovulatory period. In Specific Aim 4, the temporal expression of
mRNA for PGF2alpha receptor in bovine periovulatory follicles will be
determined. The hypothesis that mRNA for the receptor is expressed coordinately
with the rise in PG synthesis and, is localized to the theca cells of the
periovulatory follicle will be tested. Specific Aim 5 is designed to test the
hypothesis that the LWFSH surge induces progesterone receptor (PR) in
follicular cells of the periovulatory follicle and that these receptors mediate
progesterone's effects on PG production. Together these experiments will
provide new knowledge about the regulation of, and relationship between, these
two essential mediators of ovulation and their receptors.
描述(由申请人提供):LH/FSH激增启动级联反应
的事件,在卵泡壁的显着破裂达到高潮,
卵母细胞-卵丘复合体的顶端和随后的释放。尽管
对生殖和生育至关重要,尽管几十年来
研究,我们对监管的认识存在许多空白,
排卵过程许多调节器和途径被假设为
参与哺乳动物排卵,但以前的研究结果表明,
强调了孕酮和前列腺素(PGs)的重要作用。牛
提供了一个理想的动物模型,研究复杂的事件,
围排卵期我们已经验证了诱导排卵周期的模型,
牛的卵泡发育,我们现在建议用它来测试
关于这两个关键的排卵调节器及其受体的假说。在
具体目标1和2,合成的时间、细胞位点、生物合成
途径和激素调节排卵期PG合成增加
将被审查。有待检验的假设是,
细胞对排卵前LH/FSH激增的反应是PG增加,
生产,介导的激增引起的孕酮上升,这反过来又
通过还原PGE 2诱导PGF 2a合成。在第三阶段,我们将
检验孕酮对前列腺素的影响
排卵期卵泡的产生至少部分是由
导致催产素水平升高虽然PG是必不可少的
对于排卵,几乎对PG受体的表达一无所知,
在排卵期。在具体目标4中,
牛排卵期卵泡中PGF 2 α受体的mRNA将被
测定该受体的mRNA协同表达的假设
随着PG合成的增加,它定位于卵泡膜细胞,
将检测排卵期卵泡。具体目标5旨在测试
假设LWFSH激增诱导孕酮受体(PR),
排卵期卵泡的卵泡细胞,这些受体介导
孕酮对PG产生的影响。这些实验将
提供关于这些规则和关系的新知识,
排卵的两种基本介质及其受体。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JOANNE E. FORTUNE其他文献
JOANNE E. FORTUNE的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JOANNE E. FORTUNE', 18)}}的其他基金
Role of Fetal Steroids in Formation and Activation of Bovine Ovarian Follicles
胎儿类固醇在牛卵巢卵泡形成和激活中的作用
- 批准号:
7740513 - 财政年份:2009
- 资助金额:
$ 25.04万 - 项目类别:
Role of Fetal Steroids in Formation and Activation of Bovine Ovarian Follicles
胎儿类固醇在牛卵巢卵泡形成和激活中的作用
- 批准号:
7906970 - 财政年份:2009
- 资助金额:
$ 25.04万 - 项目类别:
Molecular Mechanisms of Ovarian Follicular Activation
卵巢卵泡激活的分子机制
- 批准号:
6828485 - 财政年份:2004
- 资助金额:
$ 25.04万 - 项目类别:
Regulation of Prostaglandins in Periovulatory Follicles
排卵周围卵泡中前列腺素的调节
- 批准号:
6422472 - 财政年份:2002
- 资助金额:
$ 25.04万 - 项目类别:
Regulation of Prostaglandins in Periovulatory Follicles
排卵周围卵泡中前列腺素的调节
- 批准号:
6693380 - 财政年份:2002
- 资助金额:
$ 25.04万 - 项目类别:
Regulation of Prostaglandins in Periovulatory Follicles
排卵周围卵泡中前列腺素的调节
- 批准号:
6846052 - 财政年份:2002
- 资助金额:
$ 25.04万 - 项目类别:
SELECTION OF THE DOMINANT FOLLICLE AND THE IGF SYSTEM
优势卵泡和 IGF 系统的选择
- 批准号:
6521256 - 财政年份:2000
- 资助金额:
$ 25.04万 - 项目类别:
SELECTION OF THE DOMINANT FOLLICLE AND THE IGF SYSTEM
优势卵泡和 IGF 系统的选择
- 批准号:
6637036 - 财政年份:2000
- 资助金额:
$ 25.04万 - 项目类别:
SELECTION OF THE DOMINANT FOLLICLE AND THE IGF SYSTEM
优势卵泡和 IGF 系统的选择
- 批准号:
6363441 - 财政年份:2000
- 资助金额:
$ 25.04万 - 项目类别:
SELECTION OF THE DOMINANT FOLLICLE AND THE IGF SYSTEM
优势卵泡和 IGF 系统的选择
- 批准号:
6027256 - 财政年份:2000
- 资助金额:
$ 25.04万 - 项目类别:
相似海外基金
The role of Neuregulin-1 (NRG1) in corpus luteum (CL) physiology
Neuregulin-1 (NRG1) 在黄体 (CL) 生理学中的作用
- 批准号:
10436338 - 财政年份:2020
- 资助金额:
$ 25.04万 - 项目类别:
The role of Neuregulin-1 (NRG1) in corpus luteum (CL) physiology
Neuregulin-1 (NRG1) 在黄体 (CL) 生理学中的作用
- 批准号:
10260383 - 财政年份:2020
- 资助金额:
$ 25.04万 - 项目类别:
The role of Neuregulin-1 (NRG1) in corpus luteum (CL) physiology
Neuregulin-1 (NRG1) 在黄体 (CL) 生理学中的作用
- 批准号:
10640225 - 财政年份:2020
- 资助金额:
$ 25.04万 - 项目类别:
Effects of the locational relationship between first follicular wave dominant follicle and corpus luteum on endometriumu function and embryo development
第一卵泡波优势卵泡与黄体的位置关系对子宫内膜功能及胚胎发育的影响
- 批准号:
17K15384 - 财政年份:2017
- 资助金额:
$ 25.04万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Study for the relationship between hypoxia and PG-resistance in bovine early corpus luteum -roles of BNIP3 in early corpus luteum-
牛早期黄体缺氧与PG抵抗关系的研究 -BNIP3在早期黄体中的作用-
- 批准号:
16K18803 - 财政年份:2016
- 资助金额:
$ 25.04万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Why the ipsilateral relationship where first follicular wave dominant follicle and corpus luteum are present in the same ovary decrease fertility in cattle?
为什么第一卵泡波优势卵泡和黄体存在于同一卵巢的同侧关系会降低牛的生育能力?
- 批准号:
26660234 - 财政年份:2014
- 资助金额:
$ 25.04万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Role of glycosphingolipids and galectins in the regulation of the corpus luteum function
鞘糖脂和半乳糖凝集素在黄体功能调节中的作用
- 批准号:
26460262 - 财政年份:2014
- 资助金额:
$ 25.04万 - 项目类别:
Grant-in-Aid for Scientific Research (C)