Purchase of 800 MHz NMR Spectrometer

购买800 MHz核磁共振波谱仪

• 批准号: 6441010
• 负责人: ARTHUR PARDI
• 金额: $ 50万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2005-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441010
• 关键词: biomedical equipment purchase; nuclear magnetic resonance spectroscopy; structural biology

A consortium of three Universities: The University of Colorado, Boulder, the University of Colorado Health Science Center (UCHSC) and the University of Utah School of Medicine will purchase an 800 MHZ NMR spectrometer with partial support from the NCRR Shared Instrumentation and NSF Multi-User Biological Instrumentation Program. This field NMR will benefit the research programs of the seven major users (A. Pardi and D.S. Wuttke at Boulder; W.L. Sundquist and D.R. Davis at Utah; and B.K. Bendiak, D.N.M Jones, and M.J. Overduin at UCHSC) and several minor users at the three institutions. Recent developments in TROSY-type experiments allow acquisition of extremely high resolution NMR spectra on much larger macromolecules than was previously possible. These TROSY experiments are optimized at ultra-high magnetic fields, and the requested 800 MHZ NMR will make it possible for the major users to take advantage of these powerful new techniques. The instrument will be housed in the basic science building that is part of the new campus currently being constructed for UCHSC in Denver. The major users currently have access to 500 and 600 MHZ NMR instrumentation so the requested 800 NMR will be used for projects that have a demonstrated need for a higher field, higher resolution and higher sensitivity of the 800 MHz spectrometer. The requested instrument will accelerate new and ongoing research projects in the following areas: - Oligosaccharide Structures and Cell Surface Carbohydrate-Protein Interactions- B.K. Bendiak - Structural Studies of HIV Primer/Template Complex-D.R. Davis - Structural Function Studies of Proteins and Carbohydrates Involved in Disease- D.N.M. Jones - Structures, Interactions and Dynamics of Membrane-Associated Proteins- M.J. Overduin - Structure-Function Studies of RNA-A. Pardi - Structural Studies of HIV-1 Assembly and Budding- W.I. Sundquist - Structural Studies of Biologically Important Molecular Recognition- D.S. Wuttke

由科罗拉多大学、博尔德大学、科罗拉多大学健康科学中心(UCHSC)和犹他大学医学院三所大学组成的财团将购买一台800 MHz核磁共振波谱仪，NCRR共享仪器和NSF多用户生物仪器计划将提供部分支持。这一领域的核磁共振将有利于七个主要用户(博尔德的A.Pardi和D.S.Wuttke；犹他州的W.L.Sundquist和D.R.Davis；以及UCHSC的B.K.Bendiak、D.N.M.Jones和M.J.Overduin)和三个机构的几个小用户的研究计划。TROSY型实验的最新发展使得能够获得比以前可能的更大的大分子的极高分辨率的核磁共振谱。这些TROSY实验是在超高磁场下优化的，所需的800 MHz核磁共振将使主要用户能够利用这些强大的新技术。该仪器将被安置在基础科学大楼内，这是目前正在为丹佛的UCHSC建造的新校园的一部分。主要用户目前可以使用500和600 MHz核磁共振仪器，因此所要求的800 MHz核磁共振将用于那些证明需要800 MHz光谱仪的更高磁场、更高分辨率和更高灵敏度的项目。所要求的仪器将加速下列领域的新的和正在进行的研究项目：-寡糖结构和细胞表面碳水化合物-蛋白质相互作用-B.K.本迪亚克-HIV引物/模板复合体的结构研究-D.R.戴维斯-参与疾病的蛋白质和碳水化合物的结构功能研究-D.N.M.琼斯-膜相关蛋白的结构、相互作用和动力学-M.J.RNA-A的Overduin结构-功能研究-M.J.HIV-1组装和萌发的半结构研究-WI.Sundquist-生物学重要分子识别的结构研究-D.S.Wuttke