Purchase of Leica TCS SP2 Confocal Microscope

购买 Leica TCS SP2 共焦显微镜

• 批准号: 6440390
• 负责人: Patricia Wadsworth
• 金额: $ 32.33万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440390
• 关键词: biomedical equipment purchase; confocal scanning microscopy

DESCRIPTION (provided by applicant): A group of researchers at the University of Massachusetts at Amherst proposes to purchase a Leica TCS SP-2 confocal microscope system. The new confocal microscope will replace an outmoded 14-year old BioRad 600/1000 instrument which cannot support the critical needs of the group for multi-wavelength excitation, time-resolved live cell imaging, and fluorescence recovery after photobleaching (FRAP). The instrument will be housed in the Central Microscopy Facility, a fee-based shared facility for the life sciences research community which receives a generous subsidy from the university administration in support of two full-time professional microscopists and instrument maintenance. One of the staff members has been responsible for the present confocal microscope since 1994, and has extensive skills and experience in optics, digital imaging, and computer interfaces. He will be responsible for the new Leica confocal, both in terms of user training and maintenance. As further commitment to the Facility, the Vice Chancellor for Research will fund a new technical position, whose major duties will include day-to-day instrument care and assisting confocal users following their initial training. The components of the new instrument have been selected to meet the specific needs of a core of 8 major and 7 minor users, 11 of whom have current NIH support. A Leica DM IRBE inverted microscope has been chosen for optimal microinjection capability, and the HeNe and Ar laser combination will provide the needed range of excitation wavelengths. Projects requiring advanced confocal imaging in support of the specific aims of NIH awards include: cytoskeletal dynamics during mitosis, the sorting of proteins in melanoma cells, the development of neuromuscular junctions, the mechanism of programmed cell death in Tlymphocytes, and the role of the actin cytoskeleton in pollen tube growth.

描述（由申请人提供）：一组研究人员在大学 位于阿默斯特的马萨诸塞州的一家公司提议购买一台徕卡TCS SP-2共焦显微镜， 显微镜系统新的共焦显微镜将取代过时的14年 旧的BioRad 600/1000仪器，无法支持 用于多波长激发、时间分辨活细胞成像的组，以及 光漂白后荧光恢复（FRAP）。仪器将 中央显微镜设施，一个收费的共享设施， 生命科学研究界，它得到了慷慨的补贴， 大学行政部门支持两名全职专业人员 显微镜和仪器维护。其中一名工作人员被 自1994年以来负责现有的共聚焦显微镜，并拥有广泛的经验 在光学、数字成像和计算机接口方面的技能和经验。他 我将负责新的徕卡共焦，无论是在用户培训方面 和维护作为对该基金的进一步承诺， 研究将资助一个新的技术职位，其主要职责将包括 日常仪器护理和协助共聚焦用户在他们的初始 训练 新仪器的组成部分已选定，以满足特定的 8个主要用户和7个次要用户的核心需求，其中11人目前拥有NIH 支持.选择Leica DM IRBE倒置显微镜进行最佳 微注入能力，HeNe和Ar激光组合将提供 所需的激发波长范围。需要先进的项目 共聚焦成像支持NIH奖项的具体目标包括： 有丝分裂过程中的细胞骨架动力学，黑色素瘤中蛋白质的分选 细胞，神经肌肉接头的发育，程序化的机制， T淋巴细胞中的细胞死亡，以及花粉中肌动蛋白细胞骨架的作用 管生长