Integrated Robotics for Microarray Assembly (IRMA)

微阵列组装集成机器人 (IRMA)

• 批准号: 6441113
• 负责人: CHARLES P. EMERSON
• 金额: $ 39.16万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441113
• 关键词: biomedical equipment purchase; biotechnology; developmental genetics; microarray technology; robotics

This application requests instrumentation for genome scale assembly of custom cDNA and genomic libraries for microarray transcript profiling analysis and for functional and genetic screens for model organisms. This instrumentation provides an integrated clone assembly system, referred to as Integrated Robotics for Microarray Assembly (IRMA), including: 1) a Robotic Colony Picker for recovering and assembling large libraries of bacterial and yeast clones; 2) an Incubating Growth Chamber for small volume, high density growth of large clone collections in a 96-well format; 3) a Liquid Handling Robot for large scale DNA purification and assembly of PCR amplification reactions; and 4) a High Capacity Thermocycler for PCR amplifications for sequence analysis and microarray target production. The IRMA instrumentation will be housed in the University of Pennsylvania Microarray Core Facility, under the supervision of Core lab personnel, providing an efficient and productive interface with the technical expertise and downstream instrumentation available in the Core Facility for clone management and microarray applications. The eight participating investigators have well-funded research programs investigating genetic regulation of complex developmental processes, including stem cell specification, body plan patterning, and axon guidance, using a variety of model organisms, including Mouse, Zebra fish, Chick, Drosophila, and Arabidopsis. This IRMA instrumentation will be an essential component of these projects, allowing investigators to assemble the large set of clones to pursue genome-scale functional and expression-based screens that are required to define the gene networks that mediate these developmental processes.

该应用要求用于定制cDNA和基因组文库的基因组规模组装的仪器，用于微阵列转录谱分析和用于模式生物的功能和遗传筛选。该仪器提供了一种集成克隆组装系统，称为微阵列组装集成机器人（IRMA），包括：1）用于回收和组装细菌和酵母克隆的大型文库的机器人菌落挑选器; 2）用于96孔格式的大型克隆集合的小体积、高密度生长的孵育生长室; 3）用于大规模DNA纯化和组装PCR扩增反应的液体处理机器人;和4）用于序列分析和微阵列靶产生的PCR扩增的高容量热循环仪。IRMA仪器将被安置在宾夕法尼亚大学微阵列核心设施中，在核心实验室人员的监督下，为克隆管理和微阵列应用提供与核心设施中可用的技术专业知识和下游仪器的高效和富有成效的接口。八名参与研究人员拥有资金充足的研究计划，调查复杂发育过程的遗传调控，包括干细胞规格，身体计划模式和轴突指导，使用各种模式生物，包括小鼠，斑马鱼，小鸡，果蝇和拟南芥。这种IRMA仪器将是这些项目的重要组成部分，使研究人员能够组装大量克隆，以进行基因组规模的功能和基于表达的筛选，这些筛选是定义介导这些发育过程的基因网络所必需的。