Integrated LC/MS/MS System

质谱技术的进步为蛋白质的分析提供了前所未有的机会，如肽内部测序、翻译后修饰、非共价相互作用和蛋白质折叠。在这里，我们请求资金购买集成TheroFinnigan LCQ DECA-10000/LC Packings Ultimate LC/MS/MS系统，该系统将允许新泽西州医学院和罗格斯大学纽瓦克医学和牙科大学的研究社区利用这种最先进的技术进行蛋白质研究。目前，还没有仪器可以让研究人员在这两个研究所使用串联质谱测序和阐明蛋白质结构。提供这种技术将大大提高两个校区的研究质量和生产力。我们已经召集了一组NIH资助的研究人员，加上两所大学7个部门的7名其他研究人员，他们的研究将通过我们的新泽西医学院-RUTGERS大学，纽瓦克（NJMS-RUN）质谱核心设施的集成系统的可用性得到显着增强。本申请中概述的项目需要对来自各种生物体-人类、酵母和细菌的蛋白质的结构和功能进行基础研究。将服务的研究领域同样广泛，包括蛋白质鉴定，核蛋白质复合物鉴定，蛋白质-配体相互作用，设计师金属蛋白质合成的质量控制和蛋白质的翻译后修饰。该设备将位于我们的质谱核心设施实验室。它将由负责该设施的经验丰富的质谱师Hong Li博士监督和维护。从两个机构抽调的两个委员会监督该设施的财政和科学管理以及日常运作。该设施提供的服务满足了大量需求。集成的ThermoFinnigan LCQ DECA-10000/LC Packings Ultimate LC/MC/MS系统的增加将补充我们现有的MALDI-TOF仪器，使该设施能够满足我们大部分的质谱需求。

Advances in mass spectrometry have provided unprecedented opportunities for the analysis of proteins, such as peptide internal sequencing, post-translational modification, non-covalent interaction, and protein folding. Here we request funds to purchase an integrated TheroFinnigan LCQ DECA-10000/LC Packings Ultimate LC/MS/MS system that will allow the research communities at the University of Medicine and Dentistry of New Jersey-New Jersey Medical School and Rutgers University-Newark to take advantage of this state-of-the-art technology in protein research. Currently, there is no instrument available that allows researchers at either institute to sequence and elucidate protein structures using tandem mass spectrometry. Provision of this technology will significantly enhance the quality and productivity of the research on both campuses. We have assembled a group of NIH-funded investigators plus 7 other researchers frm 7 departments at both universities whose research would be significantly enhanced by the availability of this integrated system to our New Jersey Medical School-RUTGERS University, Newark (NJMS- RUN) Mass Spectrometry Core Facility. The projects outlined in this application entail basic research into the structure and function of proteins from a wide-range of organisms-human, yeast and bacteria. The research fields that will be served are equally broad and include protein identification, nuclear protein complex identification, protein-ligand interaction, quality control of designer metallo-protein synthesis and post-translational modification of proteins. This equipment will be located in our Mass Spectrometry Core Facility laboratory. It will be supervised and maintained by Dr. Hong Li, an experienced mass spectrometrist who is in charge of the facility. Two committees drawn from both institutions oversee the fiscal and scientific management and daily operation of this facility. The service provided by this facility has met with overwhelming demand. The addition of the integrated ThermoFinnigan LCQ DECA-10000/LC Packings Ultimate LC/MC/MS system will complement our existing MALDI-TOF instrument, permitting the facility to cover most of our mass spectrometry needs.