Structured RNAs Binding to Proteins Containing the dsRBD

描述（由申请人提供）：双链RNA结合结构域（dsRBD）存在于所有生命领域，特别是在高等真核生物中。越来越多的证据表明，含有dsRBDs的蛋白质（又称dsRBPs）在基本的分子和细胞功能、感染和免疫以及发育中起着关键作用。dsRBP功能的一个关键共同主题是通过dsrbd与结构化RNA配体结合。虽然完美双链dsRNAs通常被用作体外结合伙伴，但最近的研究表明，内源性RNA配体的多样性要大得多。然而，迄今为止，这些生物相关的体内RNA配体仅在零星和小规模实验中进行了检查，导致数据不完整。本课题拟在多种常用细胞系中建立dsRBDs/dsRBPs RNA配体深度测序的实验方法，并通过计算探索其序列、结构特征和生物网络。

DESCRIPTION (provided by applicant): The double-stranded RNA binding domain (dsRBD) is present in all kingdoms of life, particularly in higher eukaryotes. Growing lines of evidence indicate that proteins containing dsRBDs, a.k.a. dsRBPs, play pivotal roles in essential molecular and cellular functions, infection and immunity, and development. A key common theme of dsRBP functions is binding to structured RNA ligands via dsRBDs. While perfectly duplexed dsRNAs are typically employed as binding partners in vitro, recent studies have indicated that endogenous RNA ligands are much more diverse. However, to date, these biologically relevant in vivo RNA ligands have been examined only sporadically and in small-scale experiments, resulting in fragmentary data. In this proposal, we plan to develop experimental methods for deep sequencing RNA ligands of dsRBDs/dsRBPs in a number of commonly used cell lines and to computationally explore their sequence and structure features and biological networks.