Colony Picker and Capillary Sequencer

菌落挑选器和毛细管测序仪

• 批准号: 6441116
• 负责人: James John Russo
• 金额: $ 9.8万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441116
• 关键词: biomedical equipment purchase; high throughput technology; nucleic acid sequence

The ability of many investigators to carry out large-scale analyses has become more dependent on automation, including colony pickers, DNA purification robots, and capillary sequencers. We have assembled a diverse group of major users with world class credentials at Columbia University's College of Physicians & Surgeons, who have particular needs for high-throughput colony picking and sequencing. Their projects cover a wide range of cutting edge research: bacterial genome sequencing and analysis; methylation patterns in the human genome; genetic studies in yeast and bacteria; new techniques for generating full-length cDNA libraries; analysis of the T cell receptor repertoire in autoimmune diseases; genetics and molecular pharmacology of heart disease; and genome studies related to learning in Aplysia. The instruments will be housed in the Sequencing and Chemical Biology Laboratory of the Columbia Genome Center, whose investigators have substantial experience in operating these and similar pieces of equipment. A plan is described for effective sharing of the instruments among all the major users, and an Internal Advisory Committee has been formed to oversee the day-to-day operation of the instruments, and to determine rules for access by additional outside users. The Genome Center is committed to supporting these instruments over the long term, as part of its effort to provide both much needed support for faculty within and outside the Center, and in developing high-throughput methods for the eventual establishment of on-campus clinical diagnostic sequencing and mutation analysis.

许多研究人员进行大规模分析的能力越来越依赖于自动化，包括菌落挑选器、DNA 纯化机器人和毛细管测序仪。我们在哥伦比亚大学内科医生和外科医生学院聚集了一批具有世界级资质的多元化主要用户，他们对高通量菌落挑选和测序有特殊需求。他们的项目涵盖广泛的前沿研究：细菌基因组测序和分析；人类基因组中的甲基化模式；酵母和细菌的遗传学研究；生成全长 cDNA 文库的新技术；自身免疫性疾病中 T 细胞受体库的分析；心脏病的遗传学和分子药理学；以及与海兔学习相关的基因组研究。这些仪器将安置在哥伦比亚基因组中心的测序和化学生物学实验室，该实验室的研究人员在操作这些设备和类似设备方面拥有丰富的经验。制定了一项计划，以便在所有主要用户之间有效共享这些工具，并成立了一个内部咨询委员会来监督这些工具的日常运行，并确定其他外部用户的访问规则。基因组中心致力于长期支持这些仪器，作为其努力的一部分，为中心内外的教师提供急需的支持，并开发高通量方法，以最终建立校园临床诊断测序和突变分析。