A HIGH PRESSURE FREEZER FOR ELECTRON MICROSCOPY USES

用于电子显微镜用途的高压冷冻机

• 批准号: 6441082
• 负责人: GINA E SOSINSKY
• 金额: $ 19.2万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441082
• 关键词: biomedical equipment purchase; electron microscopy; freezing

DESCRIPTION (provided by applicant): The purpose of this grant request is to obtain funds to purchase and install a High Pressure Freezer (HPF) machine. This unit will be used to prepare well-preserved specimens for tomography applications. High pressure freezing delivers the best specimen preparation method for preserving cell and tissue ultrastructure and therefore, obtaining high resolution in situ data. The requested equipment is a Bal-Tec 11PM 010 High Pressure Freezing Machine (HFP) and accessories for sample preparation. Our research programs in structural biology involve the three-dimensional reconstruction of cells and tissues using thick sections and intermediate voltage electron microscopy (IVEM). We coordinate and integrate our structural studies with data obtained from molecular microscopy, X-ray crystallography, biochemical, biophysical and neurophysiological techniques. Our goal is to accurately reconstruct the structure of cells and organelles using the best preparative, microscopy and computational methods. At present, the closest HFP units are located in the San Francisco Bay Area or the Tucson, Arizona. The HFP will be housed in the National Center for Microscopy and Imaging Research at the University of California, San Diego. The five major programs requiring the use of the HFP are: (1) Three-dimensional cellular organization of gap junction membrane channels (Sosinsky, P.!.); (2) Structural studies of cellular components in the Node of Ranvier (Ellisman, P.1.); (3) Changes in macromolecular associations in cerebellar Purkinje neuron dendritic spines during synaptic-depression (Tsien and Lev-Ram, P.1?s); (4) Mechanisms of non-enveloped virus entry (Johnson and Schneemann, P.I.?s); and (5) Three-dimensional structure and function of mitochondria as viewed by electron tomography (Frey, P.1.). In addition, four collaborative projects from the NCMIR Research Resource that would immediately benefit from the usage of the HFP are described briefly. These nine research projects involve over 25 investigators that would depend heavily on the use of the HPF. In all of these research projects, the primary goal is to understand the three-dimensional structure of organized cellular components and relate this morphology to their functionality.

描述（由申请人提供）：本赠款申请的目的是 获得资金购买和安装一台高压冷冻机（HPF）。 该装置将用于制备保存完好的标本，以便进行断层扫描 应用.高压冷冻提供最佳的标本制备 保存细胞和组织超微结构的方法， 高分辨率原位数据。要求的设备是Bal-Tec 11 PM 010 用于样品制备的高压冷冻机（HFP）和附件。 我们在结构生物学的研究计划涉及三维 使用厚切片和中间体重建细胞和组织 电压电子显微镜（IVEM）。我们协调和整合我们的结构 利用分子显微镜，X射线晶体学， 生物化学、生物物理学和神经生理学技术。我们的目标是 使用最好的方法准确地重建细胞和细胞器的结构， 制备，显微镜和计算方法。目前，最接近的HFP 单位位于旧金山弗朗西斯科湾区或图森，亚利桑那州。的HFP 将被安置在国家显微镜和成像研究中心 位于圣地亚哥的加州大学。五大计划要求 HFP的用途是： (1)缝隙连接膜通道的三维细胞结构 （Sosinsky，P.！.）; (2)结节细胞成分的结构研究 Ranvier（Ellisman，P.1.）; (3)大分子缔合物的变化 小脑浦肯野神经元树突棘在突触抑制（钱 和Lev-Ram，P.1？s）;（4）无包膜病毒进入的机制（约翰逊和 私家侦探施尼曼（5）三维结构和功能 通过电子断层扫描观察线粒体（Frey，P.1.）。此外，四个 NCMIR研究资源的合作项目，将立即 从使用的HFP的好处进行了简要说明。这九项研究 项目涉及25名以上的调查员，这在很大程度上取决于使用 的HPF。在所有这些研究项目中，首要目标是了解 有组织细胞成分的三维结构和相关的 这种形态与它们的功能性。