Association of Caries and Salivary Analytes

龋齿和唾液分析物协会

• 批准号: 6564063
• 负责人: GURRINDER S BEDI
• 金额: $ 11.56万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564063
• 关键词: Streptococcus mutans; benzoates; clinical research; dental caries; disease /disorder proneness /risk; enzyme linked immunosorbent assay; epidemiology; human tissue; laboratory mouse; laboratory rabbit; mucins; oral health; pathologic process; saliva; technology /technique development; tissue /cell culture

Dental caries is a multi-factorial disease in which the virulence of microbes in the plaque biofilm, salivary function and diet and nutrition play important roles. We hypothesize that salivary components of both mammalian and non-mammalian origin are associated with risk for the development of dental caries. It is speculated that no single salivary component is important than the others, rather each of these components interact in a concerted fashion and a cumulative effect is important in protecting against caries. We will develop sandwich enzyme-linked immunoassays (ELISA) to quantitate the levels of salivary mucins MG1 and MG2; acidic proline- rich proteins 1 and 3, basic proline-rich proteins PRB-1 and PRB-2; glycosylated proline-rich protein PRG; cystatins S and SN; statherin; histatins 1, 3 and 5; lysozyme; lactoferrin and peroxidase; microbial enzymes glucosyltransferase and glucosyltransferase from Streptococcus mutans; and food preservative benzoate in a large number of subjects. We have already accumulated more than 4000 samples of resting whole saliva. The data obtained will be analyzed for the intra-subject stability of the analyte levels; the cross-sectional association of the decay, missing, filled surfaces (DMFS) index of the subjects analyte levels; and the longitudinal association of the DMFS with salivary analyte levels. The comprehensive survey and the cluster analysis of multiple salivary components should generate a useful data set to test for the association of these components with the caries experience and to identify parameters that will indicate increased risk for dental caries. This study will also provide useful information to determine whether there is a clinical relationship between benzoate levels in saliva and caries experience.

龋病是一种多因素疾病，其中菌斑生物膜中微生物的毒力、唾液功能以及饮食和营养起着重要作用。我们假设哺乳动物和非哺乳动物来源的唾液成分与龋齿的发展风险相关。据推测，没有哪一种唾液成分比其他成分更重要，而是这些成分中的每一种都以协调一致的方式相互作用，累积效应对于预防龋齿很重要。我们将开发夹心酶联免疫测定法（ELISA）来定量唾液粘蛋白MG 1和MG 2;酸性富脯氨酸蛋白1和3;碱性富脯氨酸蛋白PRB-1和PRB-2;糖基化富脯氨酸蛋白PRG;半胱氨酸蛋白酶抑制剂S和SN; statherin;组蛋白1、3和5;溶菌酶;乳铁蛋白和过氧化物酶;来自变形链球菌的微生物酶葡糖基转移酶和葡糖基转移酶;和食品防腐剂苯甲酸盐。我们已经收集了4000多份静息全唾液样本。将分析获得的数据的分析物水平的受试者内稳定性;受试者分析物水平的衰减、缺失、填充表面（DMFS）指数的横截面相关性;以及DMFS与唾液分析物水平的纵向相关性。多个唾液成分的综合调查和聚类分析应该产生一个有用的数据集，以测试这些成分与龋齿经验的关联，并确定参数，将表明龋齿的风险增加。这项研究也将提供有用的信息，以确定是否有苯甲酸盐水平之间的临床关系，唾液和龋齿的经验。