Association of Caries and Salivary Analytes

龋病是一种多因素疾病，菌斑生物膜中微生物的毒力、唾液功能和饮食营养在其中起着重要作用。我们假设，哺乳动物和非哺乳动物来源的唾液成分都与龋齿的发生风险有关。据推测，唾液中没有任何一种成分比其他成分更重要，相反，这些成分中的每一种都以协调的方式相互作用，累积的效果在预防龋齿方面很重要。我们将建立双抗体夹心法检测唾液粘蛋白Mg1和Mg2、酸性富含Pro蛋白1和3、碱性富含Pro蛋白Prb-1和Prb-2、糖基化富含Pro蛋白Prg、半胱氨酸S和SN、他汀类药物、组蛋白1、3和5、溶菌酶、乳铁蛋白和过氧化物酶、变形链球菌的微生物酶葡萄糖转移酶和葡萄糖转移酶，以及食物防腐剂苯甲酸盐的含量。我们已经积累了4000多个休息全唾液样本。将对获得的数据进行分析，以确定分析物水平的受试者内部稳定性；受试者分析物水平的衰减、缺失、填充表面(DMF)指数的横截面关联；以及DMF与唾液分析物水平的纵向关联。对多种唾液成分的全面调查和聚类分析应该产生一个有用的数据集，以测试这些成分与龋齿经验的关联，并确定将表明龋齿风险增加的参数。这项研究还将提供有用的信息，以确定唾液中的苯甲酸盐水平与龋齿经历之间是否存在临床关系。

Dental caries is a multi-factorial disease in which the virulence of microbes in the plaque biofilm, salivary function and diet and nutrition play important roles. We hypothesize that salivary components of both mammalian and non-mammalian origin are associated with risk for the development of dental caries. It is speculated that no single salivary component is important than the others, rather each of these components interact in a concerted fashion and a cumulative effect is important in protecting against caries. We will develop sandwich enzyme-linked immunoassays (ELISA) to quantitate the levels of salivary mucins MG1 and MG2; acidic proline- rich proteins 1 and 3, basic proline-rich proteins PRB-1 and PRB-2; glycosylated proline-rich protein PRG; cystatins S and SN; statherin; histatins 1, 3 and 5; lysozyme; lactoferrin and peroxidase; microbial enzymes glucosyltransferase and glucosyltransferase from Streptococcus mutans; and food preservative benzoate in a large number of subjects. We have already accumulated more than 4000 samples of resting whole saliva. The data obtained will be analyzed for the intra-subject stability of the analyte levels; the cross-sectional association of the decay, missing, filled surfaces (DMFS) index of the subjects analyte levels; and the longitudinal association of the DMFS with salivary analyte levels. The comprehensive survey and the cluster analysis of multiple salivary components should generate a useful data set to test for the association of these components with the caries experience and to identify parameters that will indicate increased risk for dental caries. This study will also provide useful information to determine whether there is a clinical relationship between benzoate levels in saliva and caries experience.