Core--Molecular virology

描述(改编自应用程序)。分子病毒学核心将 有三个不同的功能来支持对人类肝细胞的研究 丙型肝炎病毒复制与肝细胞损伤的培养模型。的三大功能 分子病毒学的核心是：1)从感染性克隆中提供丙型肝炎病毒RNA 感染性来源和感染者来源的病毒源血清 研究；2)表征丙型肝炎病毒在人肝细胞模型中的复制 丙型肝炎病毒基因组和反基因组生产的定量评估；以及3)跟踪 异源双链在肝细胞培养中扩增丙型肝炎病毒准种变异株 迁移率分析和核苷酸测序。这些函数还包括 应用程序的其他部分的组件，在这方面 分子病毒学核心将是一个服务设施。此外，还有几个关键 丙型肝炎病毒感染性和肝细胞损伤病毒决定因素的假说 将会被探索。

Description (adapted from the application). The Molecular Virology Core will have three distinct functions to support research on the human hepatocyte cell culture model of HCV replication and liver cell injury. The three functions of the Molecular Virology Core are: 1) to provide HCV RNA from infectious clone sources and viremic sera from infected patient sources for use in infectivity studies; 2) to characterize HCV replication in the human hepatocyte model by quantitative assessment of HCV genome and antigenome production; and 3) track propagation of HCV quasispecies variants in hepatocyte culture by heteroduplex mobility analysis and nucleotide sequencing. These functions are also components of the other sections of the application, and in this regard the Molecular Virology Core will be a service facility. In addition, several key hypotheses on HCV infectivity and viral determinants of hepatocellular injury will be explored.