Core--Molecular virology

核心--分子病毒学

• 批准号: 6659987
• 负责人: David Richard Gretch
• 金额: $ 21.22万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6659987
• 关键词: biomedical facility; cooperative study; genetic strain; hepatitis C virus; serology /serodiagnosis; tissue /cell culture; virus RNA; virus replication

Description (adapted from the application). The Molecular Virology Core will have three distinct functions to support research on the human hepatocyte cell culture model of HCV replication and liver cell injury. The three functions of the Molecular Virology Core are: 1) to provide HCV RNA from infectious clone sources and viremic sera from infected patient sources for use in infectivity studies; 2) to characterize HCV replication in the human hepatocyte model by quantitative assessment of HCV genome and antigenome production; and 3) track propagation of HCV quasispecies variants in hepatocyte culture by heteroduplex mobility analysis and nucleotide sequencing. These functions are also components of the other sections of the application, and in this regard the Molecular Virology Core will be a service facility. In addition, several key hypotheses on HCV infectivity and viral determinants of hepatocellular injury will be explored.

描述（改编自应用程序）。分子病毒学核心将 有三种不同的功能来支持对人类肝细胞的研究， HCV复制和肝细胞损伤的培养模型。三大功能 分子病毒学核心是：1）从感染性克隆中提供HCV RNA 来源和来自感染患者来源的病毒血血清，用于感染性 研究; 2）通过以下方法表征人肝细胞模型中的HCV复制： HCV基因组和反基因组产生的定量评估;和3）跟踪 通过异源双链体在肝细胞培养物中增殖HCV准种变异体 迁移率分析和核苷酸 测序 这些功能也是 本申请其他部分的组成部分，在这方面， 分子病毒学中心将是一个服务设施。 此外，几个关键 丙型肝炎病毒感染性和肝细胞损伤病毒决定因素假说 将被探索。