CORE--IMMUNOANALYSIS

核心--免疫分析

• 批准号: 6652761
• 负责人: JAMES L MOHLER
• 金额: $ 20.83万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-26 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6652761
• 关键词: androgen receptor; apoptosis; biomedical facility; cell proliferation; histology; histopathology; immunocytochemistry; immunofluorescence technique; in situ hybridization; monoclonal antibody; prostate neoplasms; video microscopy

The Immunoanalysis Core will serve as a resource to the three Projects for 1) processing, storage and sectioning of research tissues; 2) confirmation of tissue histology using routine H&E staining; 3) analysis of apoptosis using TdT-mediated dUTP-X nick end labeling (TUNEL) technique and ApopTag detection, proliferation using MIB-1 detection of Ki-67 antigen, BrdU incorporation and androgen receptor using monoclonal antibodies by standard descriptive immunohistochemistry and quantitative video color image analysis; 4) characterization of cell cycle regulatory factors, steroid receptor co-activators, general transcription galactosidase activity for cellular marking and detection of adeno-associated viral infection and 6) androgen receptor gene amplification using in situ hybridization. Drs. Mohler and Smith will co- direct the facility and supervise the activities of individuals who already possess high levels of expertise and extensive experience in performing these functions. The Core will draw upon resources currently available as well as centralize and enhance tissue processing capability through purchase of a modest quality of equipment.

免疫分析核心将作为以下三个项目的资源：1)研究组织的处理、存储和切片；2)使用常规H&E染色确认组织组织学；3)使用TdT介导的dUTP-X缺口末端标记(TUNEL)技术和ApopTag检测细胞凋亡；使用MIB-1检测Ki-67抗原的增殖；通过标准描述性免疫组织化学和定量视频彩色图像分析，使用单抗检测BrdU掺入和雄激素受体；4)细胞周期调节因子、类固醇受体共激活因子、用于细胞标记和检测腺相关病毒感染的一般转录半乳糖苷酶活性的表征，以及6)雄激素受体基因的原位杂交扩增。莫勒博士和史密斯博士将共同领导该设施，并监督已经拥有高水平专业知识和丰富经验的个人执行这些职能的活动。核心中心将利用现有资源，并通过购买质量适中的设备集中和加强组织处理能力。