ImmunoAnalysis and Research Specimen Management

免疫分析和研究样本管理

• 批准号: 7963221
• 负责人: JAMES L MOHLER
• 金额: $ 17.39万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7963221
• 关键词: Acinus organ component; Androgen Metabolism; Androgen Receptor; Androgens; Antibodies; Apoptosis; Archives; Biochemical; Biological Markers; Bromodeoxyuridine; Clinical; Data Analyses; Elongation Factor; Enzymes; Evaluation; Freezing; Genitourinary system; Growth Factor; Histology; Human; Human Glandular Kallikrein 2; Human Resources; Image; Image Analysis; Immunohistochemistry; In Situ; Individual; Ki-67 Antigen; Lasers; Lead; Malignant neoplasm of prostate; Manuscripts; Measurement; Methods; Monoclonal Antibodies; Neurons; Paper; Paraffin Embedding; Pathology; Process; Proteins; Publications; Publishing; Recurrence; Research; Research Personnel; Services; Specimen; Staining method; Stains; Stem cells; Steroid Receptors; Thioredoxin; Tissue Microarray; Tissues; Translations; Tubulin; Urinary Retention; Visual; Xenograft procedure; angiogenesis; enolase; interest; men; programs; protein expression; receptor; tumor

Core B wiil provide 3 primary funcfions to the Program Project. First, Core B will provide research specimens to the investigators and assist with identificafion, immunostaining and image analysis. Paraffin-embedded sections will be provided from original tumors or tissue microarrays and used to 1) confirm tissue histology using roufine H&E; 2) analyze apoptosis using ACINUS, proliferation using MIB-1 detecfion of Ki-67 or BrdU incorporation and androgen receptor using monoclonal antibodies by standard, descriptive immunohistochemistry and quanfitative analysis using visual scoring and/or automated image analysis; and 3) idenfify and quanfify steroid receptor co-regulators, androgen metabolism enzymes, androgen-regulated gene products (PSA, Nkx3.1, a-tubulin, translation elongation factor la, non-neuronal enolase, tomoregulin, thioredoxin reducatase 1, Mxi-1 and human kallikrein 2), general transcripfion factors, growth factors and their receptors and stem cell and angiogenesis biomarkers using immunohistochemistry and image analysis. Second, Core B will be responsible for storage, processing and sectioning of clinical (frozen, archival and TMAs) and prostate cancer xenograft (CWR22) research specimens. Core personnel will procure, process and store clinical specimens of castrafion-recurrent prostate cancer obtained from men with advanced prostate cancer who present with urinary retention due to local recurrence. Research specimens will be used to construct tissue microarrays or laser capture microdissected for biochemical measurements of fissue androgens. Lastly, Core B will provide expert biostatistical (Dr. Wilding) and genitourinary pathological service (Dr. Alexiev) to the Program Project. Drs. Mohler and Smith will co-direct the facility and supervise the acfivifies of individuals who already possess high levels of expertise and currently perform these funcfions for the Program Project. Drs. Wilding and Alexiev will lead the efforts to integrate biostatistical and pathological expertise into Core B and the 3 projects. The high level of funcfion of Core B is demonstrated best by Core B's publicafion of 8 methods papers. Core B continues to develop new methods for more accurate measurement of protein expression (4 publicafions) and remains a leader in the use of TMAs for CaP research (2 publications). Core B has performed services that have resulted in manuscripts published, in press and submitted for Project 1 (20), Project 2 (8) and Project 3 (8). Core B also facilitates interaction among the Projects; 12 of 23 publications involved more than 1 Project.

核心B将为该计划项目提供三项主要职能。首先，核心B将向调查人员提供研究标本，并协助鉴定、免疫染色和图像分析。石蜡包埋切片将从原始肿瘤或组织微阵列提供，用于1)使用常规HE确认组织学；2)使用ACINUS分析细胞凋亡，使用MIB-1检测Ki-67或BrdU掺入的增殖，使用标准的描述性免疫组织化学和使用视觉评分和/或自动图像分析的定量分析，使用单抗分析雄激素受体；3)用免疫组织化学和图像分析方法鉴定和定性类固醇受体辅调节因子、雄激素代谢酶、雄激素调节基因产物(PSA、Nkx3.1、α-微管蛋白、翻译延伸因子1a、非神经元烯醇化酶、TomoRegin、硫氧还蛋白还原酶1、MXI-1和人激肽释放酶2)、一般转录因子、生长因子及其受体、干细胞和血管生成生物标志物。 第二，Core B将负责临床(冷冻、档案和TMA)和前列腺癌异种移植(CWR22)研究样本的储存、处理和切片。核心人员将获取、加工和存储从患有晚期前列腺癌的男性患者那里获得的复发前列腺癌的临床标本，这些男性患者因局部复发而出现尿滞留。研究样本将被用来构建组织微阵列或激光捕获显微解剖，用于裂解雄激素的生化测量。最后，Core B将为该计划项目提供生物统计学专家(Wilding博士)和泌尿生殖系统病理学专家(Alexiev博士)。 莫勒博士和史密斯博士将共同领导该设施，并监督已经 拥有高水平的专业知识，目前正在为计划项目履行这些职能。Wilding博士和Alexiev博士将领导将生物统计学和病理学专业知识整合到核心B和3个项目中的努力。核心B的高水平功能在核心B的8篇方法论文中得到了最好的展示。核心B继续开发更准确地测量蛋白质表达的新方法(4个出版物)，并在使用TMA进行CAP研究方面保持领先地位(2个出版物)。核心B为项目1(20)、项目2(8)和项目3(8)提供了出版、印刷和提交手稿的服务。核心B还促进项目之间的互动；23份出版物中有12份涉及一个以上的项目。