Homeostatic regulation of ion currents and neuronal net
Homeostatic regulation of ion currents and neuronal net
批准号:
6666858
负责人:
JORGE P GOLOWASCH
金额:
$20.02万
依托单位国家:
美国
项目类别:
财政年份:
2001
资助国家:
美国
项目状态:
已结题
起止时间:
2001-12-01 至 2006-11-30
关键词:
Malacostraca RNA animal tissue basal ganglia electrophysiology homeostasis ions learning long term potentiation membrane channels memory microelectrodes neural information processing neural plasticity neuroregulation organ culture pyloric region sensory feedback transfection voltage /patch clamp voltage gated channel
中文摘要
描述(由申请人提供):本项目旨在阐明
允许神经网络产生稳定输出的细胞机制,同时
保持应对扰动的灵活性,例如发生的扰动
在成长、学习、感官输入和损伤期间。离子流产生了
表征神经元活动的电学变化,以及单个神经元
而神经网络负责行为的产生。在苏中
在这种情况下,神经系统及其部分通常能够保持内稳态
在受到干扰或损坏后恢复它们的性能。几种机制
允许表达这种稳定或恢复活动的过程,
其中研究最多的可能是突触的可塑性。然而,一个不同的
机制正在成为表达的有力候选者
这两个看似自相矛盾的方面是神经元活动的灵活性和
稳定性。这种机制是对电压敏感的活性依赖的调节。
离子流。
在这个方案中,描述了一系列实验来研究其性质
这一鲜为人知的神经元活动调节机制。这
机制可能非常重要,因为它可能是一种新形式的
学习和记忆通过其对神经网络活动的稳定作用。这个
对小的和特征良好的神经网络的活动的调节,如
以及长期依赖于活性的离子电流调节的作用
在这一过程中将被调查。以前的工作已经显示出一些证据表明
不同离子流之间的长期相互作用和相互调节。
我将深入调查这些相互监管的相互作用及其
在稳定神经元活动的过程中可能起到作用。这个
实验方法将包括几种电生理模式
在体外记录完整网络中的神经元
生活在自由活动的动物中,在培养中的分离细胞中,在
长期的全网文化。药理操作也是如此
作为最近克隆的离子通道的RNA注射,将被用于修饰
内源性离子电流的表达。
英文摘要
DESCRIPTION (provided by applicant): This project aims to elucidate the
cellular mechanisms that allow a neural network to produce stable output while
retaining the flexibility to respond to perturbations, such as those that occur
during growth, learning, sensory input and injury. Ionic currents produce the
electrical changes that characterize neuronal activity, and individual neurons
and neural networks are responsible for the generation of behavior. In so
doing, the nervous system and its parts are often capable of homeostatically
restoring their properties after a perturbation or damage. Several mechanisms
allow this process of stabilization or restoration of activity to be expressed,
the best studied of which is perhaps synaptic plasticity. However, a different
mechanism is emerging as a powerful candidate to underlie the expression of
these two seemingly paradoxical aspects of neuronal activity of flexibility and
stability. This mechanism is activity-dependent regulation of voltage-sensitive
ionic currents.
In this proposal a series of experiments is described to study the properties
of this poorly understood mechanism of neuronal activity regulation. This
mechanism is potentially of great importance as it may underlie a new form of
learning and memory via its stabilizing effect on neural network activity. The
regulation of activity in a small and well-characterized neural network, as
well as the role of long-term activity-dependent regulation of ionic currents
in this process will be investigated. Previous work has shown some evidence of
long-term interactions and mutual regulation between different ionic currents.
I will investigate in depth these mutual regulatory interactions and their
possible role in the process of stabilization of neuronal activity. The
experimental methodology will include several modes of electrophysiological
recording of neurons in the intact network in vitro, in the intact network in
the living a freely moving animal, in dissociated cells in culture, and in
long-term culture of the whole network. Pharmacological manipulations, as well
as RNA injections of recently cloned ionic channels, will be used to modify the
expression of endogenous ionic currents.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Ionic conductance correlations tune neuronal network activity to natural inputs
-
批准号:8928696
-
项目类别:
-
资助金额:$38.27万
-
财政年份:2014
-
负责人:JORGE P GOLOWASCH
-
依托单位:
Homeostatic regulation of ion currents and neuronal net
-
批准号:6422371
-
项目类别:
-
资助金额:$7.97万
-
财政年份:2001
-
负责人:JORGE P GOLOWASCH
-
依托单位:
Role of neuromodulators and activity in the regulation of ionic currents and neur
-
批准号:8245171
-
项目类别:
-
资助金额:$26.51万
-
财政年份:2001
-
负责人:JORGE P GOLOWASCH
-
依托单位:
Role of neuromodulators and activity in the regulation of ionic currents and neur
-
批准号:7585596
-
项目类别:
-
资助金额:$28.14万
-
财政年份:2001
-
负责人:JORGE P GOLOWASCH
-
依托单位:
Neuromodulation of Ionic Currents and Neuronal Network Activity
-
批准号:8434281
-
项目类别:
-
资助金额:$25.45万
-
财政年份:2001
-
负责人:JORGE P GOLOWASCH
-
依托单位:
Homeostatic regulation of ion currents and neuronal net
-
批准号:6620836
-
项目类别:
-
资助金额:$12.19万
-
财政年份:2001
-
负责人:JORGE P GOLOWASCH
-
依托单位:
Homeostatic regulation of ion currents and neuronal net
-
批准号:6986177
-
项目类别:
-
资助金额:$17.04万
-
财政年份:2001
-
负责人:JORGE P GOLOWASCH
-
依托单位:
Role of neuromodulators and activity in the regulation of ionic currents and neur
-
批准号:8051631
-
项目类别:
-
资助金额:$26.51万
-
财政年份:2001
-
负责人:JORGE P GOLOWASCH
-
依托单位:
Role of neuromodulators and activity in the regulation of ionic currents and neur
-
批准号:7828199
-
项目类别:
-
资助金额:$26.75万
-
财政年份:2001
-
负责人:JORGE P GOLOWASCH
-
依托单位:
Homeostatic regulation of ion currents and neuronal net
-
批准号:6818103
-
项目类别:
-
资助金额:$17.49万
-
财政年份:2001
-
负责人:JORGE P GOLOWASCH
-
依托单位:
Homeostatic regulation of ion currents and neuronal net
-
批准号:6687753
-
项目类别:
-
资助金额:$20.02万
-
财政年份:2001
-
负责人:JORGE P GOLOWASCH
-
依托单位:
国内基金
海外基金
登录
查看更多内容
基于合成生物标志物的超多重RNA数字化检测平台用于肿瘤精准诊断和分期评估
-
批准号:
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2026
-
负责人:程子译
-
依托单位:
RNA m6A修饰通过调控FDX1介导的铜死亡参与补阳还五汤抗脑缺血再灌注损伤作用机制的研究
-
批准号:2026JJ81091
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2026
-
负责人:刘亮
-
依托单位:
免标记CRISPR-RNA适配体与门逻辑分子诊断新方法研究
-
批准号:2026JJ50010
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2026
-
负责人:应站明
-
依托单位:
Dead-box解旋酶DDX23通过调控RNA高级结构促进肝癌细胞恶性生物学行为的分子机制研究
-
批准号:JCZRLH202600588
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2026
-
负责人:
-
依托单位:
基于基因编辑技术解析丹酚酸B靶向SAMHD1调控心肌线粒体RNA稳态干预心衰的分子机制研究
-
批准号:JCZRLH202601084
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2026
-
负责人:
-
依托单位:
RNA 结合蛋白HuR与VEGF-D联合调控舌鳞癌侵袭及转移机制的研究
-
批准号:2026JJ80684
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2026
-
负责人:龚攀
-
依托单位:
基于异质人群多源数据识别单细胞 RNA数量性状风险位点的统计学方法研究
-
批准号:
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2026
-
负责人:蔡铭轩
-
依托单位:
uN2CpolyG蛋白经ALYREF蛋白介导RNA转运异常在神经元核内包涵体病发病中的作用及机制研究
-
批准号:2026JJ60587
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2026
-
负责人:张思哲
-
依托单位:
病毒非编码RNA多样性图谱构建及其生物发生与致病机制研究
-
批准号:2026JJ60389
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2026
-
负责人:傅萍
-
依托单位:
核糖核酸酶RNase E与其抑制因子RebA通过液-液相分离调控蓝藻RNA代谢的分子机制
-
批准号:JCZRQNB202600879
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2026
-
负责人:
-
依托单位: