Identification of drug resistance genes in Fasciola hepatica using a combined in vitro phenotyping and pooled genotyping approach

使用体外表型和汇总基因分型相结合的方法鉴定肝片形吸虫的耐药基因

• 批准号: 2270039
• 金额: --
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2270039
• 关键词: Identification; drug; resistance; genes; Fasciola

Fasciola hepatica, the common liver fluke is a major cause of disease and production loss in sheep and cattle in the UK and changes in farming practices and climate are leading to increased prevalence and movement of infection into new areas of the UK. To control liver fluke we rely on repeated use of anthelmintic drugs (flukicides), particularly, triclabendazole, to which widespread drug-resistance is now reported. The concern is that as farmers turn to other drugs such as closantel, that resistance will now emerge to these drugs that are currently effective in the majority of cases. The aim of this project is to use in vitro methods to screen parasites displaying resistance to these alternative flukicides and genetically determine what genes are responsible so that we can better understand how parasites become resistant but also so we can detect and pre-empt the development of multi-drug resistance by providing best practice advice on anthelmntic use to farmers. In addition to developing high level genomic and bioinformatics skills, you will work directly with farmers. This project would be ideal for a qualified veterinary surgeon, or biology graduate with an interest in veterinary science, who wishes to develop skills in advanced next generation sequencing.BBSRC's Strategic Priorities include an emphasis on 'Agriculture and food security' and specifically: improving the UK's capacity to produce sufficient affordable nutritious safe food. This project falls clearly within that remit because it aims to provide evidence-based advice on parasite control in sheep and cattle and therefore improve meat and milk production. By achieving better control of parasites, promoting strategic guidance on judicious use of anthelmintics whilst identifying the emergence of drug resistance in the field, this project will improve animal welfare, feed conversion and nutrition of sheep and cattle, making meat and milk production more efficient, sustainable and cost-effective; contributing directly to national GDP. The proposal addresses several priority areas within agriculture and food security, such as: animal health, welfare of managed animals, and enhancing sustainable agricultural production. The student will gain experience in working with farmers, demonstrating the high impact of this research, also a target for BBSRC.

肝片形吸虫是一种常见的肝吸虫，是英国绵羊和牛疾病和产量损失的主要原因，农业实践和气候的变化导致感染率增加，并进入英国的新地区。为了控制肝吸虫，我们依赖于反复使用驱虫药（杀吸虫剂），特别是三氯苯达唑，目前已报告了广泛的耐药性。令人担忧的是，随着农民转向其他药物，如closantel，现在将出现耐药性，这些药物目前在大多数情况下是有效的。该项目的目的是使用体外方法筛选对这些替代杀吸虫剂具有抗性的寄生虫，并从遗传学上确定哪些基因负责，以便我们能够更好地了解寄生虫如何产生抗性，同时也可以通过向农民提供驱虫剂使用的最佳实践建议来检测和预防多药耐药性的发展。除了培养高水平的基因组学和生物信息学技能外，您还将直接与农民合作。这个项目将是理想的合格兽医，或生物学毕业生与兽医科学的兴趣，谁希望发展先进的下一代测序的技能。BBSRC的战略重点包括强调“农业和粮食安全”，特别是：提高英国的能力，以生产足够的负担得起的营养安全食品。该项目福尔斯显然属于这一范围，因为它旨在提供关于羊和牛寄生虫控制的循证咨询意见，从而提高肉类和牛奶产量。通过更好地控制寄生虫，促进对驱虫药明智使用的战略指导，同时确定该领域出现的耐药性，该项目将改善动物福利，饲料转化和羊和牛的营养，使肉类和牛奶生产更有效，可持续和具有成本效益;直接促进国家GDP。该提案涉及农业和粮食安全的几个优先领域，例如：动物健康、管理动物的福利以及加强可持续农业生产。学生将获得与农民合作的经验，展示这项研究的高影响力，这也是BBSRC的目标。