RESEARCH CENTER FOR CEREBRAL VASCULAR DISEASE

脑血管疾病研究中心

• 批准号: 6539517
• 负责人: MYRON DAVID GINSBERG
• 金额: $ 149.27万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-08-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6539517
• 关键词: cerebral ischemia /hypoxia; neuroprotectants

The objective of the Cerebral Vascular Disease Research Center at the University of Miami is to carry out an integrated, multi-disciplinary program of experimental research into the mechanisms and therapies of ischemic brain injury. The overall goal of our research program is to identify and characterize those factors responsible for engendering ischemic injury that may be amenable to therapeutic or prophylactic intervention. The Program Project consists of three investigative proposals supported by General-Scientific and Image- Analysis/Computing Core facilities. Project areas include 1) focal cerebral ischemia: a study of the mechanisms of neuroprotection underlying human serum albumin therapy, and of exacerbation of injury due to hyperthermia (fever); 2) mitochondria and cerebral anoxia/ischemia: basic mechanistic studies of protection and dysfunction; and 3) the metabolic and structural consequences of evolving mitochondrial and ionic dysfunction in focal ischemia. The projects are closely integrated with one another, both conceptually and methodologically, and are strongly supported by close collaborative interactions among the investigators and research personnel. The core facilities contribute substantively to the implementation of each project and unify the overall program by common methodological approaches. The General Scientific Core compromises animal physiology; methods of focal and global cerebral ischemia; neurobehavioral strategies; morphological methods including quantitative light microscopy and stereology; immunohistochemistry, confocal microscopy; autoradiographic strategies for local cerebral blood flow and glucose utilization; enzymatic tissue sampling and metabolic analyses; and molecular biological methods, including in situ hybridization, Northern and Western blotting, and reverse transcription-polymerase chain reaction (RT-PCR). The Image-Analysis and Computing Core has pioneered in the development of three-dimensional autoradiographic image-averaging and is a central resource supporting image-analysis of autoradiographic, molecular biological, and histopathological data; as well as acquisition, statistical analysis and archiving of laboratory data, instrumentation control, and data base management.

迈阿密大学脑血管疾病研究中心的目标是对缺血性脑损伤的机制和治疗进行综合的、多学科的实验研究。我们研究计划的总体目标是识别和描述那些可能导致缺血性损伤的因素，这些因素可能适合于治疗性或预防性干预。该方案项目由三项调查提案组成，并得到通用科学和图像分析/计算核心设施的支持。项目领域包括1)局灶性脑缺血：人血清白蛋白治疗的神经保护机制研究，以及高温(发烧)导致的损伤加重机制的研究；2)线粒体和脑缺氧/缺血：保护和功能障碍的基本机制研究；3)局灶性脑缺血中线粒体和离子功能障碍演变的代谢和结构后果的研究。这些项目在概念和方法上彼此紧密结合，并得到调查人员和研究人员之间密切协作互动的大力支持。核心设施为每个项目的实施作出了实质性贡献，并通过共同的方法方法统一了整个方案。通用科学核心包括动物生理学；局灶性和全局性脑缺血的方法；神经行为策略；形态方法，包括定量光学显微镜和体视学；免疫组织化学、共聚焦显微镜；局部脑血流和葡萄糖利用的放射自显影策略；酶组织采样和代谢分析；以及分子生物学方法，包括原位杂交、Northern和Western blotting以及逆转录聚合酶链式反应(RT-PCR)。图像分析和计算核心是开发三维放射自显影图像平均的先驱，是支持放射自显影、分子生物学和组织病理学数据的图像分析，以及实验室数据的获取、统计分析和存档、仪器控制和数据库管理的中央资源。