STRUCTURE OF DNA POLYMERASE OF HYPERTHERMOPHILE PYROCOCCUS FURIOSUS (PFU)

超嗜热火球菌 (PFU) 的 DNA 聚合酶的结构

• 批准号: 6586617
• 负责人: LI-WEI HUNG
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586617
• 关键词: bacteria; bioimaging /biomedical imaging; biomaterials; biomedical resource; enzymes; genome; proteins; radiography; structural biology

DNA polymerase is the enzyme responsible for the replication of the genome and repair of DNA molecule in cells. Both mesophilic and thermophilic polymerase proteins have been solved but no whole polymerase from a hyperthermophile has been completed. The structural data from a solved crystal would give more data with which to improve their understanding of the polymerase structure with respect to its activities such as DNA binding the two exonuclease activities and polymerase activity. The structure may help in expanding our basic understanding of intramolecular interaction which contribute to the thermostability of this hyperthermophilic protein.

DNA聚合酶是负责复制的酶。 基因组和DNA分子的修复。 既嗜中温， 嗜热聚合酶蛋白已被解决，但没有完整的 一种嗜热菌的聚合酶已经完成了 结构性 来自溶解晶体的数据将提供更多的数据， 他们对聚合酶结构的理解， 活性如DNA结合两种外切核酸酶活性， 聚合酶活性 这种结构可能有助于扩大我们的基本 分子内相互作用的理解，这有助于 这种超嗜热蛋白的热稳定性。