CORE--CELL SCIENCE

核心--细胞科学

• 批准号: 6590771
• 负责人: KENNETH S.K. TUNG
• 金额: $ 17.42万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6590771
• 关键词: apoptosis; bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; cytology; digital imaging; electron microscopy; fluorescence microscopy; histology; histopathology; image processing; immunocytochemistry; in situ hybridization; messenger RNA; method development; protein localization; single cell analysis; tissue /cell preparation; tissue resource /registry

The Cell Science Core (CSC) is an integral part of the U54 Center, providing services to its investigators and to the reproductive biology researchers at the University of Virginia by funded by the NICHD RBS Branch. The main goal of the CSC is to accomplish projects that rely on tissue and cellular localization of antigenic molecules and messenger RNA (mRNA), as well as morphologic analysis of pathologic tissue and cellular changes. Dr. Kenneth Tung, a human and experimental pathologist with expertise in reproduction and immunology, will continue to direct the CSC. He will consult or collaborate on studies requiring preparation of high quality tissue sections suitable for histology, immunohistology, immunocytology, in situ hybridization and apoptotic cell detection, as well as tissue and cellular localization of antigenic molecules and interpretation of histopathology of animal tissues. He will work with Dr. Mark Stoler, Co-director, a pathologist and an expert in situ hybridization who will consult or collaborate on studies requiring mRNA detection and quantitation. They will organize, prioritize, and quality- control the work of Ms. Melissa Bevard, an experienced histologist and Ms. Mary McCanna, an in situ hybridization technologist. When appropriate, they will implement the transfer to investigators' laboratories techniques through training of researchers, technicians, and monitoring of their work. The CSC will also develop new technology in response to and in anticipation of, research needs that will evolve in the U54. Examples include: 1) double-antibody labeling technique and double-antibody/in situ labeling technique, 2) confocal microscopy, 3) acquisition of reagents and techniques for human translational and murine reproduction research. External consultants have been recruited to serve the CSC to support this endeavor. The Core will be adapted to meet the changing research needs of the new U54 context. The P30-CSC unit has successfully provided quality service to a growing number of reproductive biology researcher, responsible for the completion of over 50 research projects and acquisition of numerous NIH grant proposals.

细胞科学核心（CSC）是U 54中心的一个组成部分， 为研究人员和生殖生物学提供服务 弗吉尼亚大学的研究人员由NICHD RBS资助， 分支。CSC的主要目标是完成依赖于 抗原分子和信使RNA的组织和细胞定位 （mRNA）以及病理组织和细胞的形态学分析 变化Kenneth Tung博士是一位人类和实验病理学家， 在生殖和免疫学的专业知识，将继续指导CSC。 他将咨询或合作研究需要准备高 适用于组织学，免疫组织学， 免疫细胞学、原位杂交和凋亡细胞检测， 以及抗原分子的组织和细胞定位， 动物组织的组织病理学解释。他将与博士。 Mark Stallow，联合主任，病理学家和原位专家 杂交谁将咨询或合作的研究需要mRNA 检测和定量。他们会组织，优先，和质量- 控制梅利莎贝瓦德女士的工作，一位经验丰富的组织学家和女士。 原位杂交技术专家玛丽·麦坎纳。在适当的时候， 他们将把技术转移到研究人员的实验室， 通过培训研究人员、技术人员和监测其 工作CSC还将开发新技术，以应对 预测，研究需求将在U 54发展。示例 包括：1）双抗体标记技术和双抗体/原位 标记技术，2）共聚焦显微镜，3）试剂的获取， 用于人类翻译和小鼠生殖研究的技术。 已聘请外部顾问为CSC提供支持， 奋进。核心将进行调整，以满足不断变化的研究需求， 新U 54的背景。P30-CSC装置成功地提供了 为越来越多的生殖生物学研究人员提供服务， 负责完成50多个研究项目， 获得了大量的NIH拨款提案。