CORE--ANIMAL

说明：动物核心的主要功能是提供维生素a 用于项目2-4的耗尽（VAD）同步测试。芯会 负责维生素消耗、视黄醇替代和大鼠分期。 核心将执行同步测试的评估和验证， 保持老鼠实验的记录。大鼠将由以下人员圈养和护理： 霍普金斯动物设施和预算的很大一部分反映了 老鼠的每日护理费用。动物核心目录估计 只有70%的VAD大鼠会同步。项目1将 使用70只大鼠，项目2和项目3将共用动物， 每年24只老鼠分离Sertoli所需的大鼠数量 估计每年有148只老鼠。考虑到百分之三十的失败率 VAD同步率，估计每年所需的大鼠数量 将是352。

Description: The major function of the animal core is to provide vitamin a depleted (VAD) synchronized testes for Projects 2-4. The core will be responsible for vitamin depletion, retinol replacement, and staging of rats. The core will perform assessment and verification of synchronized testes, and maintain records of rat experiments. The rats will be housed and cared for by the Hopkins animal facility and a significant portion of the budget reflects the per diem care costs for the rats. The animal core directory estimates that only 70% of the VAD rats will become synchronized. Project 1 will utilize 70 rats, and projects 2 and 3 will share animals and utilize a total of 24 rats per year. The number of rats required for isolation of Sertoli cells is estimated to be 148 rats per year. Considering a 30 percent failure rate in VAD synchronization, the estimated number of rats required per year will be 352.