CORE--HIGH RESOLUTION MICROSCOPY

核心——高分辨率显微镜

• 批准号: 6605460
• 负责人: KENT T KEYSER
• 金额: $ 19.59万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-02 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6605460
• 关键词: bioimaging /biomedical imaging; biomedical equipment resource; biomedical facility; confocal scanning microscopy; digital imaging

Description: (Applicant's Description) The detection and imaging of molecules in biological systems is an increasingly important tool in biomedical research. Confocal microscopy has become a leading technology for this purpose. In 1998, Dr. Kent Keyser was the recipient of an NIH NCRR Shared Instrument Grant, a UAB Health Services Foundation award, and start-up funds from the UAB Office of the Provost, the Chairs of Departments in both the Medical and Academic Divisions of UAB, and Center Directors. These funds were used to establish a new facility, the High Resolution Imaging Facility. This imaging resource is equipped with two Leica Confocal Imaging Spectrophotometer TCS SP units. One is configured for multiphoton excitation with a Coherent MIRA Ti: Sa ultrafast laser with output selectable between 690-990 nanometers This permits imaging of living cells and tissues with minimal phototoxicity, and of structures 200-400 micrometers from the tissue surface. The SP scan head provides an individual spectrophotometer for each detector channel, allowing selection of specific portions of the emission spectrum for delivery to the detector. The result is higher fluorescence yield, reduced crosstalk, and improved image quality. The Facility also includes an Olympus Fluoview CLSM with fiber launched Krypton ion and Argon ion lasers, conventional fluorescence and DIC microscopy equipment, and work stations equipped with digital deconvolution software, output and image storage devices. In addition, through a cooperative agreement with the Birmingham Veteran?s Administration Medical Center, Cancer Center members will have access to a Leica TCS SP unit equipped with a UV laser and UV corrected optics. All three Leica instruments are equipped with either three or four detector channels, a transmitted light channel, and Argon ion, Krypton ion and Helium: Neon lasers. These systems are able to image up to three fluorophores simultaneously and are capable of obtaining DIC images as well. The software packages offer three-dimensional reconstruction, time-lapse imaging, ratio imaging, physiology packages, and morphometry routines. The Facility will offer Comprehensive Cancer Center members access to a state-of-the-art imaging facility with a full range of instrumentation and the expertise to use these resources productively.

描述：（申请人的描述）分子的检测和成像 在生物系统中是生物医学中越来越重要的工具 研究。共焦显微镜已成为这方面的领先技术 目的。 1998 年，Kent Keyser 博士获得 NIH NCRR 共享仪器 补助金、UAB 健康服务基金会奖以及 UAB 的启动资金 教务长办公室、医学和各部门的主席 UAB 学术部门和中心主任。这些资金被用于 建立一个新设施，即高分辨率成像设施。这种成像 资源配备两台徕卡共焦成像分光光度计TCS SP 单位。一种配置为使用相干 MIRA Ti 进行多光子激发： Sa 超快激光器，输出波长可在 690-990 纳米之间选择 允许以最小的光毒性对活细胞和组织进行成像，并且 距组织表面200-400微米的结构。 SP扫描头 为每个检测器通道提供单独的分光光度计，允许 选择发射光谱的特定部分以传送到 探测器。结果是更高的荧光产量、更少的串扰，以及 提高图像质量。该设施还包括奥林巴斯 Fluoview CLSM 使用光纤发射的氪离子和氩离子激光器，传统 荧光和 DIC 显微镜设备以及配备以下设备的工作站 数字反卷积软件、输出和图像存储设备。在 此外，通过与伯明翰退伍军人协会的合作协议 行政医疗中心、癌症中心会员将有机会获得 Leica TCS SP 装置配备紫外激光器和紫外校正光学器件。全部三个 徕卡仪器配备三个或四个检测器通道，一个 透射光通道，以及氩离子、氪离子和氦：氖激光器。 这些系统能够同时对多达三个荧光团进行成像 也能够获得 DIC 图像。软件包提供 三维重建、延时成像、比率成像、 生理学包和形态测量程序。该设施将提供 综合癌症中心成员可以获得最先进的成像 拥有全套仪器设备和使用这些仪器的专业知识的设施 有效地利用资源。