SIVMAC239 INFECTION IN SOOTY MANGABEYS & RHESUS MACAQUES

乌白眉猴中的 SIVMAC239 感染

• 批准号: 6591319
• 负责人: A KAUR
• 金额: $ 11.11万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6591319
• 关键词: AIDS; Mammalia; communicable diseases; immunology; inflammation; lymphatic system; technology /technique; virus

Sooty mangabeys naturally infected with simian immunodeficiency virus (SIV) do not develop immunodeficiency despite the presence of viral loads of 105 to 107 RNA copies/ml To investigate the basis of apathogenic SIV infection in sooty mangabeys, three sooty mangabeys and three rhesus macaques were inoculated intravenously with SIVmac239 and evaluated longitudinally for one year SIVmac239 infection of sooty mangabeys resulted in 2 to 4 log lower viral loads than in macaques and did not reproduce the high viral loads observed in natural SIVsmm infection During acute SIV infection, polyclonal CTL activity coincident with decline in peak plasma viremia was observed in both macaques and mangabeys Eight to 20 weeks later, CTL activity declined in the macaques but was sustained and broadly directed in the mangabeys Neutralizing antibodies to SIVmac239 were detected in the macaques but not the mangabeys Differences in expression of CD38 on CD8+ T lymphocytes or in t he percentage of naive phenotype T cells expressing CD45RA and CD62L-selectin did not correlate with development of AIDS in rhesus macaques In macaques, the proportion of CD4+ T lymphocytes expressing CD25 declined during SIV infection, while in mangabeys, CD25-expressing CD4+ T lymphocytes increased Longitudinal evaluation of cytokine secretion by flow cytometric analysis of unstimulated lymphocytes revealed elevation of IL-2 and interferon gamma in a macaque and only IL-10 in a concurrently infected mangabey during acute SIV infection Differences in host responses following experimental SIVmac239 infection may be associated with the divergent outcome in sooty mangabeys and rhesus macaques REFERENCES Kaur A, Grant RM, Means RE, McClure H, Feinberg M, Johnson RP Diverse host response and outcome following SIVmac239 infection in sooty mangabeys and rhesus macaques J Virol 1998; 72:9597-9611

黑白眉猴自然感染猿免疫缺陷 病毒（SIV）不发展免疫缺陷，尽管存在 病毒载量为105至107 RNA拷贝/ml 三只白眉猴的SIV感染 3只恒河猴静脉接种SIVmac 239 并纵向评估了一年SIVmac 239感染的患者， 白眉猴的病毒载量比猕猴低2 - 4个对数 并且没有再现在天然SIVsmm中观察到的高病毒载量 感染在急性SIV感染期间， 与血浆病毒血症峰值下降一致， 猕猴和白眉猴8到20周后，CTL活性下降， 在猕猴中，但在 在猴中检测到SIVmac 239的中和抗体。 猕猴而不是白眉猴CD 38表达的差异 CD 8 + T淋巴细胞或初始表型T细胞百分比 表达CD 45 RA和CD 62 L-selectin与 在猕猴中， 表达CD 25的CD 4 + T淋巴细胞在SIV感染过程中下降， 白眉猴表达CD 25的CD 4 + T淋巴细胞增加， 流式细胞术对细胞因子分泌的纵向评价 未刺激的淋巴细胞分析显示IL-2升高， 猕猴中的干扰素γ和同时的仅IL-10 SIV急性感染过程中感染的白眉猴宿主差异 实验性SIVmac 239感染后的反应可能与 在白眉猴和恒河猴中的结果不同 Kaur A，Grant RM，Means RE，麦克卢尔H，Feinberg M，约翰逊 RP感染SIVmac 239后的不同宿主反应和结局 白眉猴和恒河猴J病毒学1998; 72：9597-9611