SIVMAC239 INFECTION IN SOOTY MANGABEYS & RHESUS MACAQUES

自然感染猿猴免疫缺陷的煤烟曼加比 病毒(SIV)不会发展为免疫缺陷，尽管存在 病毒载量为105至107个RNA拷贝/毫升，以调查基础 三只煤烟鸟中无致病性SIV感染的研究 对3只恒河猴静脉接种SIVmac239 并对烟尘SIVmac239感染进行了一年的纵向评估 黑尾猴的病毒载量比猕猴低2到4个对数 并且没有复制在天然SIVsmm中观察到的高病毒载量 急性SIV感染期间的感染，多克隆CTL活性 与血浆病毒血症峰值下降相一致的是，在这两个患者中都观察到了 8到20周后，猕猴和短尾猴的CTL活性下降 在猕猴身上，但持续和广泛地指导在 检测到芒果鼠对SIVmac239的中和抗体 猕猴CD38的表达差异不明显 CD8 T淋巴细胞或幼稚表型T细胞的百分比 CD45RA和CD62L-选择素的表达与 艾滋病在恒河猴中的发展，在猕猴中的比例 表达CD25的CD4T淋巴细胞在SIV感染过程中下降， 而在芒果鼠中，表达CD25的CD4T淋巴细胞增加 流式细胞术对细胞因子分泌的纵向评价 未经刺激的淋巴细胞分析显示IL-2和 猕猴体内的干扰素-γ和IL-10同时存在 急性SIV感染期间感染芒果的宿主差异 实验性SIVmac239感染后的反应可能与 在煤烟猕猴和恒河猴身上的不同结果 参考文献Kaur A，Grant RM，Means RE，McClure H，Feinberg M，Johnson SIVmac239感染后的不同宿主反应和转归 烟灰黑尾猴和恒河猴J Virol 1998；72：9597-9611

Sooty mangabeys naturally infected with simian immunodeficiency virus (SIV) do not develop immunodeficiency despite the presence of viral loads of 105 to 107 RNA copies/ml To investigate the basis of apathogenic SIV infection in sooty mangabeys, three sooty mangabeys and three rhesus macaques were inoculated intravenously with SIVmac239 and evaluated longitudinally for one year SIVmac239 infection of sooty mangabeys resulted in 2 to 4 log lower viral loads than in macaques and did not reproduce the high viral loads observed in natural SIVsmm infection During acute SIV infection, polyclonal CTL activity coincident with decline in peak plasma viremia was observed in both macaques and mangabeys Eight to 20 weeks later, CTL activity declined in the macaques but was sustained and broadly directed in the mangabeys Neutralizing antibodies to SIVmac239 were detected in the macaques but not the mangabeys Differences in expression of CD38 on CD8+ T lymphocytes or in t he percentage of naive phenotype T cells expressing CD45RA and CD62L-selectin did not correlate with development of AIDS in rhesus macaques In macaques, the proportion of CD4+ T lymphocytes expressing CD25 declined during SIV infection, while in mangabeys, CD25-expressing CD4+ T lymphocytes increased Longitudinal evaluation of cytokine secretion by flow cytometric analysis of unstimulated lymphocytes revealed elevation of IL-2 and interferon gamma in a macaque and only IL-10 in a concurrently infected mangabey during acute SIV infection Differences in host responses following experimental SIVmac239 infection may be associated with the divergent outcome in sooty mangabeys and rhesus macaques REFERENCES Kaur A, Grant RM, Means RE, McClure H, Feinberg M, Johnson RP Diverse host response and outcome following SIVmac239 infection in sooty mangabeys and rhesus macaques J Virol 1998; 72:9597-9611