SIVMAC239 INFECTION IN SOOTY MANGABEYS & RHESUS MACAQUES

乌黑白眉猴自然感染猿猴免疫缺陷 尽管存在病毒（SIV），但不会出现免疫缺陷 病毒载量为 105 至 107 RNA 拷贝/ml 调查基础 乌白眉猴的非致病性 SIV 感染，三只乌白眉猴 三只恒河猴静脉注射 SIVmac239 并对煤烟的一年 SIVmac239 感染进行纵向评估 白眉猴的病毒载量比猕猴低 2 到 4 个对数 并且没有重现在天然 SIVsmm 中观察到的高病毒载量 感染 急性SIV感染期间，多克隆CTL活性 在两种情况下都观察到与峰值血浆病毒血症下降同时发生 猕猴和白眉猴 8 至 20 周后，CTL 活性下降 在猕猴中，但在 在白眉猴中检测到 SIVmac239 的中和抗体 猕猴而非白眉猴 CD38 表达差异 CD8 T 淋巴细胞或幼稚表型 T 细胞的百分比 表达 CD45RA 和 CD62L-选择素与 恒河猴中艾滋病的发展 在猕猴中， 在 SIV 感染期间表达 CD25 的 CD4 T 淋巴细胞下降， 而在白眉猴中，表达 CD25 的 CD4 T 淋巴细胞增加 通过流式细胞仪纵向评估细胞因子分泌 对未刺激淋巴细胞的分析显示 IL-2 升高， 猕猴中的干扰素 γ 和同时存在的 IL-10 急性 SIV 感染期间感染的白眉猴 宿主差异 实验性 SIVmac239 感染后的反应可能与 乌白眉猴和恒河猴的结果不同 参考文献 Kaur A、Grant RM、Means RE、McClure H、Feinberg M、Johnson RP SIVmac239 感染后的不同宿主反应和结果 乌黑白眉猴和恒河猴 J Virol 1998； 72:9597-9611

Sooty mangabeys naturally infected with simian immunodeficiency virus (SIV) do not develop immunodeficiency despite the presence of viral loads of 105 to 107 RNA copies/ml To investigate the basis of apathogenic SIV infection in sooty mangabeys, three sooty mangabeys and three rhesus macaques were inoculated intravenously with SIVmac239 and evaluated longitudinally for one year SIVmac239 infection of sooty mangabeys resulted in 2 to 4 log lower viral loads than in macaques and did not reproduce the high viral loads observed in natural SIVsmm infection During acute SIV infection, polyclonal CTL activity coincident with decline in peak plasma viremia was observed in both macaques and mangabeys Eight to 20 weeks later, CTL activity declined in the macaques but was sustained and broadly directed in the mangabeys Neutralizing antibodies to SIVmac239 were detected in the macaques but not the mangabeys Differences in expression of CD38 on CD8+ T lymphocytes or in t he percentage of naive phenotype T cells expressing CD45RA and CD62L-selectin did not correlate with development of AIDS in rhesus macaques In macaques, the proportion of CD4+ T lymphocytes expressing CD25 declined during SIV infection, while in mangabeys, CD25-expressing CD4+ T lymphocytes increased Longitudinal evaluation of cytokine secretion by flow cytometric analysis of unstimulated lymphocytes revealed elevation of IL-2 and interferon gamma in a macaque and only IL-10 in a concurrently infected mangabey during acute SIV infection Differences in host responses following experimental SIVmac239 infection may be associated with the divergent outcome in sooty mangabeys and rhesus macaques REFERENCES Kaur A, Grant RM, Means RE, McClure H, Feinberg M, Johnson RP Diverse host response and outcome following SIVmac239 infection in sooty mangabeys and rhesus macaques J Virol 1998; 72:9597-9611