Understanding the effects of Natural Variation and Disease Stress on Meiotic Recombination in Arabidopsis thaliana

了解自然变异和疾病胁迫对拟南芥减数分裂重组的影响

• 批准号: 2273478
• 金额: --
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2273478
• 关键词: Understanding; effects; Natural; Variation; Disease

PhD project strategic theme: Bioscience for sustainable agriculture and food Mapping meiotic recombination quantitative trait loci (qRTL) between Eurasian and African Arabidopsis thaliana accessions will utilise simple sequence length polymorphisms (SSLPs) to genotype different F2 individuals derived from the crossed. These crosses contain meiotic crossover reporters, meaning recombination phenotype can be compared to SSLP genotype data in order to perform a scan for QTLs influencing recombination. The direction of the project may evolve to look more closely at the centromeric regions as the project progresses, which show high levels of structural polymorphisms between accessions. Furthermore, the effect of disease and immunity signalling on meiotic recombination will be investigated. Arabidopsis plants will be infected with both natural and generalist pathogens and the next generation will be analysed for any changes in recombination frequency using fluorescent or molecular markers.

博士项目战略主题：可持续农业和食品生物科学欧亚和非洲拟南芥种质之间的减数分裂重组数量性状基因座（qRTL）作图将利用简单序列长度多态性（SSLP）对来自杂交的不同F2个体进行基因分型。这些杂交包含减数分裂交换报告基因，这意味着重组表型可以与SSLP基因型数据进行比较，以便对影响重组的QTL进行扫描。随着项目的进展，该项目的方向可能会演变为更密切地关注着丝粒区域，这些区域显示出高水平的结构多态性。此外，将研究疾病和免疫信号对减数分裂重组的影响。拟南芥植物将感染自然和generalist病原体和下一代将分析重组频率的任何变化，使用荧光或分子标记。