Characterization of the roles of PP1 phosphatases in cytokinesis

PP1 磷酸酶在胞质分裂中的作用表征

• 批准号: 2273719
• 金额: --
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2273719
• 关键词: Characterization; roles; PP1; phosphatases; cytokinesis

PhD project strategic theme: Understanding the rules of life Cell division faithfully partitions the genomic information between the two daughter cells and errors in this process have been implicated in many human diseases. Most mitotic events are regulated by phosphorylation and, although mitotic kinases have been studied is some detail, very little is known about their counteracting phosphatases. The host laboratory has recently characterised the protein interaction network in the last phase of mitosis, cytokinesis, and found that PP1 phosphatases were the most abundant and frequent. The aim of this project is to characterize the roles of these phosphatases during cytokinesis using a multi-systems approach combining gene editing, targeted proteolysis, quantitative proteomics, and multi-dimensional high resolution microscopy.

博士项目战略主题：理解生命的规则细胞分裂忠实地分割两个子细胞之间的基因组信息，这一过程中的错误已涉及许多人类疾病。大多数有丝分裂事件是由磷酸化调控的，尽管对有丝分裂激酶进行了一些详细的研究，但对它们的对抗磷酸酶知之甚少。宿主实验室最近描述了有丝分裂，细胞质分裂最后阶段的蛋白质相互作用网络，并发现PP1磷酸酶是最丰富和最频繁的。该项目的目的是利用结合基因编辑、靶向蛋白水解、定量蛋白质组学和多维高分辨率显微镜的多系统方法来表征这些磷酸酶在细胞分裂过程中的作用。