Role of Conventional Myosin MYH9 in Hearing

传统肌球蛋白 MYH9 在听力中的作用

• 批准号: 6545854
• 负责人: Anil K Lalwani
• 金额: $ 37.63万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-05 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6545854
• 关键词: actins; adenosinetriphosphatase; alleles; cell line; clinical research; congenital deafness; disease /disorder model; gene expression; gene mutation; gene targeting; genetically modified animals; genotype; growth /development; hearing; human genetic material tag; human subject; human tissue; laboratory mouse; labyrinth; molecular pathology; myosins; phenotype; protein protein interaction; protein structure function; transfection

DESCRIPTION (provided by applicant): Through the candidate gene approach, we have identified MYH9 as the causative gene responsible for DFNA17, an autosomal dominant nonsyndromic form of hereditary hearing impairment. MYH9, a conventional nonmuscle myosin, joins the growing list of myosins associated with hearing loss. In the DFNA17 family, a G to A transition at nucleotide 2114 changes codon 705 from an invariant arginine (R) to histidine (H), R705H, within a highly conserved SHI linker region. The co-segregation of the mutant MYH9 with nonsyndromic hearing impairment illustrates a biologically significant role for MYH9 in hearing and an organ-specific pathology associated with the mutant allele.The objective of the proposed research is to understand the role of MYH9 and its mutant allele MYH9R7O5H in hearing and its dysfunction. We will test the hypothesis that MYH9 is essential for normal hearing and that the mutant allele MYH9R7O5H leads to myosin dysfunction and auditory impairment. Initially, we will assess the importance of MYH9 to hearing in humans. Individuals with hearing loss of unknown genetic etiology will be screened for alterations in the MYH9 gene to determine the contribution of MYH9 mutations in nonsyndromic hearing impairment. The discovery of additional mutations will facilitate genotype-phenotype correlation and determining the contribution of MYH9 to hearing loss in general. Secondly, we will assess the role of MYH9 in inner ear development and hearing. This will be carried out by characterizing the expression pattern of Myh9 in the developing and an adult mouse inner ear and determining the effects of its absence. We will use the XA1 36 ES cell line carrying a marker gene insertion into its Myh9 allele to generate mice transgenic for the Myh9 null allele. Thirdly, we will assess the effect of the MYH9R7O5H mutation on myosin function in vitro and in vivo. MYH9R7O5H will be characterized in vitro for its ATPase activity, actin-dependent motility and the effect of its expression in cultured cell lines in which biological role of MYH9 has been established. Generating a mouse model of DFNA1 7 through targeted or random germline introduction of Myh9R7O5H allele will assess the effect of MYH9R7O5H in vivo. In summary, the proposed research will lead to elucidation of the role of MYH9 in hearing and deafness.

描述（由申请人提供）：通过候选基因方法，我们已经确定MYH9是导致DFNA17的致病基因，DFNA17是一种常染色体显性非综合征型遗传性听力障碍。MYH9，一种传统的非肌肉肌球蛋白，加入了与听力损失相关的肌球蛋白名单。在DFNA17家族中，核苷酸2114的G到a跃迁使密码子705在高度保守的SHI连接区域内从不变的精氨酸(R)变为组氨酸(H) R705H。突变体MYH9与非综合征性听力障碍的共分离说明了MYH9在听力和与突变等位基因相关的器官特异性病理中的生物学重要作用。本研究的目的是了解MYH9及其突变等位基因MYH9R7O5H在听力及其功能障碍中的作用。我们将验证MYH9对正常听力至关重要的假设，以及突变等位基因MYH9R7O5H导致肌球蛋白功能障碍和听力障碍。首先，我们将评估MYH9对人类听力的重要性。对于遗传原因不明的听力损失患者，将筛查MYH9基因的改变，以确定MYH9突变在非综合征性听力障碍中的作用。其他突变的发现将促进基因型-表型相关性，并确定MYH9对听力损失的贡献。其次，我们将评估MYH9在内耳发育和听力中的作用。这将通过表征Myh9在发育和成年小鼠内耳中的表达模式并确定其缺失的影响来实现。我们将使用携带标记基因插入其Myh9等位基因的XA1 36es细胞系来产生Myh9空等位基因的转基因小鼠。第三，我们将在体外和体内评估MYH9R7O5H突变对肌球蛋白功能的影响。MYH9R7O5H将在体外对其atp酶活性、肌动蛋白依赖的运动性及其在MYH9生物学作用已确立的培养细胞系中的表达影响进行表征。通过靶向或随机种系导入Myh9R7O5H等位基因建立小鼠dfna17模型，在体内评估Myh9R7O5H的作用。综上所述，本研究将有助于阐明MYH9在听力和耳聋中的作用。