CHEMICAL RELEASE & IMMUNE SENSITIZATION BY DENTAL RESINS

化学释放

• 批准号: 6516451
• 负责人: ANAHID JEWETT
• 金额: $ 24.07万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6516451
• 关键词: apoptosis; biopsy; biotransformation; chemical hypersensitivity; clinical research; composite resins; contact dermatitis; delayed hypersensitivity; dental pulp; dentin; drug adverse effect; excretion; fluorescence microscopy; guinea pigs; human subject; immune tolerance /unresponsiveness; immunocytochemistry; in situ hybridization; laboratory mouse; oral facial restoration material; oral mucosa; polymerase chain reaction; polymethacrylate; skin hypersensitivity; tissue /cell culture

DESCRIPTION (Adapted from investigator's Abstract): This is a competing continuation application whose overall goal is to ensure that resin-based materials which are placed onto teeth as part of dental care can continue to be used safely. Resin based materials which polymerize in the mouth are now used by dentists in many ways to help prevent tooth decay and for tooth repair and replacement. Bonding technologies, which are all based on this class of materials, are used for fissure sealing to help prevent decay, for bonding orthodontic brackets to teeth, and as part of many different types of tooth repair to improve appearance and function. It is very likely that new and modified materials of the same general type will continue to be developed and that the use of this type of material in dentistry will continue to increase in the coming years. The materials are very helpful to patients and have remarkably few negative side effects. However, severe allergic dermatitis in some dentists and other dental workers is linked to the use of the materials, and the incidence of such allergy appears to be increasing. Fortunately, allergic responses are less common in patients, but we do not know why this is so or whether it will continue to be so. Some patients experience pain in the dental pulp after resins are used in deep fillings, for reasons that may be related to direct chemical damage caused by released chemicals, by allergy to them, or to both. It has previously been shown in laboratory studies that two chemicals are released from these materials during the first days after they are placed on teeth. The first aim will be to confirm this release in experimental animals (guinea pigs), and study what happens to the chemicals in the body (their uptake, distribution, time of storage, breakdown and excretion). The second aim will be to study mechanisms of allergic responses to these chemicals at the cellular and molecular level using guinea pigs and mice. The third aim will be to determine whether there are differences between the risk of allergy with skin contact (as can occur in dental workers) relative to contact with the inside of the mouth and through tooth structure to the tooth pulp (as can occur in patients) in the same animals. The fourth aim will be to study allergic responses at the cellular and molecular level using blood and other tissues donated by volunteer dentists and other dental workers, to ensure that the experimental studies of the phenomenon using animal models and animal tissues in culture are valid. The studies described will help to prevent and treat adverse effects of this class of dental materials.

描述（改编自研究者摘要）：这是一项竞争性研究， 其总体目标是确保树脂基 作为牙齿护理的一部分放置在牙齿上的材料可以继续 安全使用。 树脂基材料，在口腔中， 牙医在许多方面使用它来帮助预防蛀牙和牙齿 修理和更换。 粘合技术，都是基于这个 一类材料，用于裂缝密封，以帮助防止腐烂， 将正畸托架粘结到牙齿上，并且作为许多不同类型的 牙齿修复，以改善外观和功能。 很有可能 相同类型的新材料和改性材料将继续被 开发和使用这种类型的材料在牙科将 在未来几年继续增加。 这些材料非常有助于 患者，并有显着很少的负面影响。 然而，严重 一些牙医和其他牙科工作者的过敏性皮炎与 材料的使用，以及这种过敏的发生率似乎 增加。 幸运的是，过敏反应在患者中不太常见， 但我们不知道为什么会这样，也不知道是否会继续这样。 有些病人在使用树脂后会感到牙髓疼痛， 深层填充，原因可能与直接化学损伤有关 由释放的化学物质引起，或对它们过敏，或两者兼而有之。 它有 先前在实验室研究中显示， 从这些材料的第一天后，他们被放置在牙齿上。 第一个目标将是在实验动物中证实这种释放 （豚鼠），并研究发生了什么变化的化学物质在体内（他们的 吸收、分布、储存时间、分解和排泄）。 第二 目的将是研究这些化学物质的过敏反应机制， 在细胞和分子水平上使用豚鼠和小鼠。 第三个目标 将是确定是否有差异的风险， 皮肤接触过敏（如可能发生在牙科工作者），相对于 与口腔内部接触并通过牙齿结构到达 牙髓（可能发生在患者身上）。 第四个目标 将在细胞和分子水平上研究过敏反应 使用志愿牙医和其他牙科医生捐赠的血液和其他组织， 工人，以确保实验研究的现象， 培养的动物模型和动物组织有效。 研究 所描述的将有助于预防和治疗这类药物的不良反应。 牙科材料。

项目成果

N-acetyl cysteine protects pulp cells from resin toxins in vivo.

N-乙酰半胱氨酸保护牙髓细胞免受体内树脂毒素的侵害。

• DOI: 10.1177/154405910808700603
• 发表时间: 2008
• 期刊: Journal of dental research
• 影响因子: 7.6
• 作者: Paranjpe,A;Sung,EC;Cacalano,NA;Hume,WR;Jewett,A
• 通讯作者: Jewett,A

Distribution and excretion of TEGDMA in guinea pigs and mice.

TEGDMA 在豚鼠和小鼠中的分布和排泄。

• DOI: 10.1177/00220345010800050501
• 发表时间: 2001
• 期刊: Journal of dental research
• 影响因子: 7.6
• 作者: Reichl,FX;Durner,J;Hickel,R;Kunzelmann,KH;Jewett,A;Wang,MY;Spahl,W;Kreppel,H;Moes,GW;Kehe,K;Walther,U;Forth,W;Hume,WR
• 通讯作者: Hume,WR

Diffusion of resin monomers through human carious dentin in vitro.

树脂单体在体外通过人龋牙本质的扩散。

• DOI: 10.1111/j.1600-9657.1997.tb00001.x
• 发表时间: 1997
• 期刊: Endodontics & dental traumatology
• 作者: Hamid,A;Hume,WR
• 通讯作者: Hume,WR

Variation in phosphoric acid concentration and treatment time and HEMA diffusion through dentin.

磷酸浓度和治疗时间的变化以及 HEMA 通过牙本质的扩散。

• 发表时间: 1996
• 期刊: American journal of dentistry.
• 作者: Hamid,A;Sutton,W;Hume,WR
• 通讯作者: Hume,WR