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Osteoblast response to strain at implant-tissue interfac

成骨细胞对种植体-组织界面应变的反应

基本信息

批准号：
6615760
负责人：
JOEL D BUMGARDNER
金额：
$ 6.99万
依托单位：
MISSISSIPPI STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-08-15 至 2005-08-14
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The overall objective of this project is to investigate the hypothesis that osteoblast function, specifically biosynthesis of extracellular matrix, and mineralization are affected by the magnitude and frequency of strain present at the implant-tissue interface. Support for this hypothesis is provided by the fact that placement of dental implant within bone will change the local biomechanical environment, which in turn will affect the healing and integration of the device. Excessive loading or non-loading of bone will result in fibrous tissue or bone resorption respectively around the implant instead of osseointegration. Since tissues grow in intimate contact with implant surfaces in vivo, it is essential to study how cells respond to implant surface strains under well characterized loading conditions and thus develop bone matrix at the tissue-implant interface. This information is critical as immediately loaded implant systems are designed. To study this hypothesis, a custom cell culture strain plate will be used to apply known magnitudes of strain and frequency of loading to osteoblast cells growing on an implant alloy in vitro. This strategy is important since it provides the means to measure and assess biomechanical interactions between cells and materials on a local level under in vivo-like conditions. The nature of such interactions cannot be determined by other means. In this two year study, the expression of osteoblast markers and production/secretion of matrix will be evaluated and compared to unstrained control cells over a five day interval. The hypothesis will be evaluated by measuring osteoblast extracellular matrix production parameters such collagen type I and osteocalcin by ELISA, and mineralization by alkaline phosphatase activity and calcium deposition by colorimetric and microscopic analyses. The significance of this study will be to provide critical information on the role that the local biomechanical environment plays in thge formaiton of the implant-tissue interface. Furthermore, it may also be useful in identifying loading parameters that promote osteointegration.

描述（由申请人提供）：该项目的总体目标是研究成骨细胞功能，特别是细胞外基质的生物合成和矿化受到植入物-组织界面处应变的幅度和频率影响的假设。支持这一假设的事实是，将牙种植体放置在骨内将改变局部生物力学环境，这反过来又会影响该装置的愈合和整合。骨的过度负载或不负载将分别导致种植体周围的纤维组织或骨吸收，而不是骨整合。由于在体内组织生长与种植体表面紧密接触，因此必须研究细胞如何在良好表征的载荷条件下对种植体表面应变作出反应，从而在组织-种植体界面形成骨基质。这一信息对于设计即刻加载的植入物系统至关重要。为了研究这一假设，将使用定制的细胞培养应变板对在体外植入物合金上生长的成骨细胞施加已知大小的应变和加载频率。这种策略很重要，因为它提供了在类似体内条件下在局部水平上测量和评估细胞与材料之间的生物力学相互作用的方法。这种相互作用的性质无法通过其他手段确定。在这项为期两年的研究中，将评价成骨细胞标志物的表达和基质的产生/分泌，并在5天的时间间隔内与未应变的对照细胞进行比较。将通过ELISA测量成骨细胞细胞外基质产生参数（如I型胶原蛋白和骨钙素）以及通过比色和显微镜分析测量碱性磷酸酶活性和钙沉积的矿化来评估该假设。本研究的意义在于提供了关于局部生物力学环境在种植体-组织界面形成中所起作用的关键信息。此外，它也可能是有用的，在确定负载参数，促进骨整合。